Versatile protein tagging in cells with split fluorescent protein
Tagging proteins with fluorescent proteins is a powerful method for both imaging and non-imaging applications. Here the authors use the eleventh β-strand of sfGFP and sfCherry as epitope tags for multicolour imaging and amplified signals by tandem arrangement; shortness of the tag enabled introducti...
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Bibliographic Details
| Main Authors: |
Daichi Kamiyama,
Sayaka Sekine,
Benjamin Barsi-Rhyne,
Jeffrey Hu,
Baohui Chen,
Luke A. Gilbert,
Hiroaki Ishikawa,
Manuel D. Leonetti,
Wallace F. Marshall,
Jonathan S. Weissman,
Bo Huang |
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| Format: | Article
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| Language: | English |
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| Published: |
Nature Portfolio
2016-03-01
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| Series: | Nature Communications
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| Online Access: | https://doi.org/10.1038/ncomms11046
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