Recyclable CRISPR/Cas9-mediated gene disruption and deletions in Histoplasma

Recyclable CRISPR/Cas9-mediated gene disruption and deletions in Histoplasma

ABSTRACTTargeted gene disruption is challenging in the dimorphic fungal pathogen Histoplasma due to the low frequency of homologous recombination. Transformed DNA is either integrated ectopically into the genome or maintained extrachromosomally by de novo addition of telomeric sequences. Based on a...

Full description

Bibliographic Details
Main Authors: Bastian Joehnk, Nebat Ali, Mark Voorhies, Keith Walcott, Anita Sil
Format: Article
Language:English
Published: American Society for Microbiology 2023-12-01
Series:mSphere
Subjects:
CRISPR
genome editing
fungal pathogenesis
Histoplasma
Online Access:https://journals.asm.org/doi/10.1128/msphere.00370-23

Internet

https://journals.asm.org/doi/10.1128/msphere.00370-23

Similar Items