Platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation.
Nitrite is a nitric oxide (NO) metabolite in tissues and blood, which can be converted to NO under hypoxia to facilitate tissue perfusion. Although nitrite is known to cause vasodilation following its reduction to NO, the effect of nitrite on platelet activity remains unclear. In this study, the eff...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3262819?pdf=render |
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| author | Sirada Srihirun Thanaporn Sriwantana Supeenun Unchern Dusadee Kittikool Egarit Noulsri Kovit Pattanapanyasat Suthat Fucharoen Barbora Piknova Alan N Schechter Nathawut Sibmooh |
| author_facet | Sirada Srihirun Thanaporn Sriwantana Supeenun Unchern Dusadee Kittikool Egarit Noulsri Kovit Pattanapanyasat Suthat Fucharoen Barbora Piknova Alan N Schechter Nathawut Sibmooh |
| author_sort | Sirada Srihirun |
| collection | DOAJ |
| description | Nitrite is a nitric oxide (NO) metabolite in tissues and blood, which can be converted to NO under hypoxia to facilitate tissue perfusion. Although nitrite is known to cause vasodilation following its reduction to NO, the effect of nitrite on platelet activity remains unclear. In this study, the effect of nitrite and nitrite+erythrocytes, with and without deoxygenation, on platelet activity was investigated.Platelet aggregation was studied in platelet-rich plasma (PRP) and PRP+erythrocytes by turbidimetric and impedance aggregometry, respectively. In PRP, DEANONOate inhibited platelet aggregation induced by ADP while nitrite had no effect on platelets. In PRP+erythrocytes, the inhibitory effect of DEANONOate on platelets decreased whereas nitrite at physiologic concentration (0.1 µM) inhibited platelet aggregation and ATP release. The effect of nitrite+erythrocytes on platelets was abrogated by C-PTIO (a membrane-impermeable NO scavenger), suggesting an NO-mediated action. Furthermore, deoxygenation enhanced the effect of nitrite as observed from a decrease of P-selectin expression and increase of the cGMP levels in platelets. The ADP-induced platelet aggregation in whole blood showed inverse correlations with the nitrite levels in whole blood and erythrocytes.Nitrite alone at physiological levels has no effect on platelets in plasma. Nitrite in the presence of erythrocytes inhibits platelets through its reduction to NO, which is promoted by deoxygenation. Nitrite may have role in modulating platelet activity in the circulation, especially during hypoxia. |
| first_indexed | 2024-12-13T13:27:12Z |
| format | Article |
| id | doaj.art-10899e0c68214247b27ccc09d6e5d8ce |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T13:27:12Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-10899e0c68214247b27ccc09d6e5d8ce2022-12-21T23:44:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0171e3038010.1371/journal.pone.0030380Platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation.Sirada SrihirunThanaporn SriwantanaSupeenun UnchernDusadee KittikoolEgarit NoulsriKovit PattanapanyasatSuthat FucharoenBarbora PiknovaAlan N SchechterNathawut SibmoohNitrite is a nitric oxide (NO) metabolite in tissues and blood, which can be converted to NO under hypoxia to facilitate tissue perfusion. Although nitrite is known to cause vasodilation following its reduction to NO, the effect of nitrite on platelet activity remains unclear. In this study, the effect of nitrite and nitrite+erythrocytes, with and without deoxygenation, on platelet activity was investigated.Platelet aggregation was studied in platelet-rich plasma (PRP) and PRP+erythrocytes by turbidimetric and impedance aggregometry, respectively. In PRP, DEANONOate inhibited platelet aggregation induced by ADP while nitrite had no effect on platelets. In PRP+erythrocytes, the inhibitory effect of DEANONOate on platelets decreased whereas nitrite at physiologic concentration (0.1 µM) inhibited platelet aggregation and ATP release. The effect of nitrite+erythrocytes on platelets was abrogated by C-PTIO (a membrane-impermeable NO scavenger), suggesting an NO-mediated action. Furthermore, deoxygenation enhanced the effect of nitrite as observed from a decrease of P-selectin expression and increase of the cGMP levels in platelets. The ADP-induced platelet aggregation in whole blood showed inverse correlations with the nitrite levels in whole blood and erythrocytes.Nitrite alone at physiological levels has no effect on platelets in plasma. Nitrite in the presence of erythrocytes inhibits platelets through its reduction to NO, which is promoted by deoxygenation. Nitrite may have role in modulating platelet activity in the circulation, especially during hypoxia.http://europepmc.org/articles/PMC3262819?pdf=render |
| spellingShingle | Sirada Srihirun Thanaporn Sriwantana Supeenun Unchern Dusadee Kittikool Egarit Noulsri Kovit Pattanapanyasat Suthat Fucharoen Barbora Piknova Alan N Schechter Nathawut Sibmooh Platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation. PLoS ONE |
| title | Platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation. |
| title_full | Platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation. |
| title_fullStr | Platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation. |
| title_full_unstemmed | Platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation. |
| title_short | Platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation. |
| title_sort | platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation |
| url | http://europepmc.org/articles/PMC3262819?pdf=render |
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