Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry

Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry

Summary: Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures.For complete details on the use and ex...

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Bibliographic Details
Main Authors: Jinyuan Song, Junlei Zhang, Yongtao Ji, Jianghui Tang, Jianpeng Sheng, Tingbo Liang, Xueli Bai
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell Isolation
Single Cell
Flow Cytometry
Cancer
Health Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723006469

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http://www.sciencedirect.com/science/article/pii/S2666166723006469

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