Optimized microwave-assisted extraction of polyphenols and tannins from Syzygium cumini (L.) Skeels leaves through an experimental design coupled to a desirability approach
Abstract The present study consisted in optimizing the extractive method of polyphenols and total tannins of leaves of Syzygium cumini (L) Skeels assisted by microwaves to potentiate the antimicrobial activity of the dried extract of S. cumini against sensitive and resistant strains. A Box-Behnken d...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2021-07-01
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| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300908&tlng=en |
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| author | CAIO C.A.R. SILVA CAMILA L. GOMES LUCAS J.A. DANDA ANA EMÍLIA M. ROBERTO ANA MARIA R. DE CARVALHO EULÁLIA C.P.A. XIMENES ROSALI M.F. DA SILVA MATHEUS A. ANGELOS LARISSA A. ROLIM PEDRO J. ROLIM NETO |
| author_facet | CAIO C.A.R. SILVA CAMILA L. GOMES LUCAS J.A. DANDA ANA EMÍLIA M. ROBERTO ANA MARIA R. DE CARVALHO EULÁLIA C.P.A. XIMENES ROSALI M.F. DA SILVA MATHEUS A. ANGELOS LARISSA A. ROLIM PEDRO J. ROLIM NETO |
| author_sort | CAIO C.A.R. SILVA |
| collection | DOAJ |
| description | Abstract The present study consisted in optimizing the extractive method of polyphenols and total tannins of leaves of Syzygium cumini (L) Skeels assisted by microwaves to potentiate the antimicrobial activity of the dried extract of S. cumini against sensitive and resistant strains. A Box-Behnken design that consisted of 27 experimental runs coupled with a desirability function for multiple response optimization was employed to optimize the total polyphenols content and total tannins content. Antimicrobial sensitivity tests were evaluated by obtaining the minimum inhibitory concentration, minimum fungicidal concentration and minimum bactericidal concentration in 96-well petri dishes. The optimal extraction conditions were found to be 8 min of extraction, under 300 w of microwave power, using a 1:34 g/mL solid/solvent ratio and 38% of ethanol concentration as extraction solvent. The parameter with the greatest influence in the extraction was primarily the time, followed by the potency and proportion solid/solvent. This yielded a total polyphenol content of 87.37 ± 1.85 mg TAE g-1ext and a total tannin content of 79.68 ± 1.64 mg TAE g-1 ext. All tested microorganisms were sensitive to the extract, evidencing the effectiveness of the extraction method optimization. |
| first_indexed | 2024-12-24T01:51:43Z |
| format | Article |
| id | doaj.art-10947e5dbb3b423093aabd4c100d90fd |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-24T01:51:43Z |
| publishDate | 2021-07-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-10947e5dbb3b423093aabd4c100d90fd2022-12-21T17:21:42ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902021-07-0193210.1590/0001-3765202120190632Optimized microwave-assisted extraction of polyphenols and tannins from Syzygium cumini (L.) Skeels leaves through an experimental design coupled to a desirability approachCAIO C.A.R. SILVAhttps://orcid.org/0000-0001-8380-8301CAMILA L. GOMEShttps://orcid.org/0000-0002-0585-7308LUCAS J.A. DANDAhttps://orcid.org/0000-0003-1526-1572ANA EMÍLIA M. ROBERTOhttps://orcid.org/0000-0003-2284-2535ANA MARIA R. DE CARVALHOhttps://orcid.org/0000-0002-3416-4244EULÁLIA C.P.A. XIMENEShttps://orcid.org/0000-0002-4486-0374ROSALI M.F. DA SILVAhttps://orcid.org/0000-0002-4139-6035MATHEUS A. ANGELOShttps://orcid.org/0000-0002-2994-7068LARISSA A. ROLIMhttps://orcid.org/0000-0002-2403-1026PEDRO J. ROLIM NETOhttps://orcid.org/0000-0003-0723-2587Abstract The present study consisted in optimizing the extractive method of polyphenols and total tannins of leaves of Syzygium cumini (L) Skeels assisted by microwaves to potentiate the antimicrobial activity of the dried extract of S. cumini against sensitive and resistant strains. A Box-Behnken design that consisted of 27 experimental runs coupled with a desirability function for multiple response optimization was employed to optimize the total polyphenols content and total tannins content. Antimicrobial sensitivity tests were evaluated by obtaining the minimum inhibitory concentration, minimum fungicidal concentration and minimum bactericidal concentration in 96-well petri dishes. The optimal extraction conditions were found to be 8 min of extraction, under 300 w of microwave power, using a 1:34 g/mL solid/solvent ratio and 38% of ethanol concentration as extraction solvent. The parameter with the greatest influence in the extraction was primarily the time, followed by the potency and proportion solid/solvent. This yielded a total polyphenol content of 87.37 ± 1.85 mg TAE g-1ext and a total tannin content of 79.68 ± 1.64 mg TAE g-1 ext. All tested microorganisms were sensitive to the extract, evidencing the effectiveness of the extraction method optimization.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300908&tlng=enAntibacterialantifungalexperimental design microwave-assisted extractionSyzigium cumini |
| spellingShingle | CAIO C.A.R. SILVA CAMILA L. GOMES LUCAS J.A. DANDA ANA EMÍLIA M. ROBERTO ANA MARIA R. DE CARVALHO EULÁLIA C.P.A. XIMENES ROSALI M.F. DA SILVA MATHEUS A. ANGELOS LARISSA A. ROLIM PEDRO J. ROLIM NETO Optimized microwave-assisted extraction of polyphenols and tannins from Syzygium cumini (L.) Skeels leaves through an experimental design coupled to a desirability approach Anais da Academia Brasileira de Ciências Antibacterial antifungal experimental design microwave-assisted extraction Syzigium cumini |
| title | Optimized microwave-assisted extraction of polyphenols and tannins from Syzygium cumini (L.) Skeels leaves through an experimental design coupled to a desirability approach |
| title_full | Optimized microwave-assisted extraction of polyphenols and tannins from Syzygium cumini (L.) Skeels leaves through an experimental design coupled to a desirability approach |
| title_fullStr | Optimized microwave-assisted extraction of polyphenols and tannins from Syzygium cumini (L.) Skeels leaves through an experimental design coupled to a desirability approach |
| title_full_unstemmed | Optimized microwave-assisted extraction of polyphenols and tannins from Syzygium cumini (L.) Skeels leaves through an experimental design coupled to a desirability approach |
| title_short | Optimized microwave-assisted extraction of polyphenols and tannins from Syzygium cumini (L.) Skeels leaves through an experimental design coupled to a desirability approach |
| title_sort | optimized microwave assisted extraction of polyphenols and tannins from syzygium cumini l skeels leaves through an experimental design coupled to a desirability approach |
| topic | Antibacterial antifungal experimental design microwave-assisted extraction Syzigium cumini |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300908&tlng=en |
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