Anti-hyperalgesic effects of photobiomodulation therapy (904 nm) on streptozotocin-induced diabetic neuropathy imply MAPK pathway and calcium dynamics modulation
Abstract Several recent studies have established the efficacy of photobiomodulation therapy (PBMT) in painful clinical conditions. Diabetic neuropathy (DN) can be related to activating mitogen-activated protein kinases (MAPK), such as p38, in the peripheral nerve. MAPK pathway is activated in respon...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-10-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-19947-2 |
| _version_ | 1797996120640585728 |
|---|---|
| author | Willians Fernando Vieira Kauê Franco Malange Silviane Fernandes de Magalhães Júlia Borges Paes Lemes Gilson Gonçalves dos Santos Catarine Massucato Nishijima Alexandre Leite Rodrigues de Oliveira Maria Alice da Cruz-Höfling Cláudia Herrera Tambeli Carlos Amilcar Parada |
| author_facet | Willians Fernando Vieira Kauê Franco Malange Silviane Fernandes de Magalhães Júlia Borges Paes Lemes Gilson Gonçalves dos Santos Catarine Massucato Nishijima Alexandre Leite Rodrigues de Oliveira Maria Alice da Cruz-Höfling Cláudia Herrera Tambeli Carlos Amilcar Parada |
| author_sort | Willians Fernando Vieira |
| collection | DOAJ |
| description | Abstract Several recent studies have established the efficacy of photobiomodulation therapy (PBMT) in painful clinical conditions. Diabetic neuropathy (DN) can be related to activating mitogen-activated protein kinases (MAPK), such as p38, in the peripheral nerve. MAPK pathway is activated in response to extracellular stimuli, including interleukins TNF-α and IL-1β. We verified the pain relief potential of PBMT in streptozotocin (STZ)-induced diabetic neuropathic rats and its influence on the MAPK pathway regulation and calcium (Ca2+) dynamics. We then observed that PBMT applied to the L4-L5 dorsal root ganglion (DRG) region reduced the intensity of hyperalgesia, decreased TNF-α and IL-1β levels, and p38-MAPK mRNA expression in DRG of diabetic neuropathic rats. DN induced the activation of phosphorylated p38 (p-38) MAPK co-localized with TRPV1+ neurons; PBMT partially prevented p-38 activation. DN was related to an increase of p38-MAPK expression due to proinflammatory interleukins, and the PBMT (904 nm) treatment counteracted this condition. Also, the sensitization of DRG neurons by the hyperglycemic condition demonstrated during the Ca2+ dynamics was reduced by PBMT, contributing to its anti-hyperalgesic effects. |
| first_indexed | 2024-04-11T10:12:28Z |
| format | Article |
| id | doaj.art-109d49297c3c47e6a95d08bb0c8c9110 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T10:12:28Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-109d49297c3c47e6a95d08bb0c8c91102022-12-22T04:30:04ZengNature PortfolioScientific Reports2045-23222022-10-0112111810.1038/s41598-022-19947-2Anti-hyperalgesic effects of photobiomodulation therapy (904 nm) on streptozotocin-induced diabetic neuropathy imply MAPK pathway and calcium dynamics modulationWillians Fernando Vieira0Kauê Franco Malange1Silviane Fernandes de Magalhães2Júlia Borges Paes Lemes3Gilson Gonçalves dos Santos4Catarine Massucato Nishijima5Alexandre Leite Rodrigues de Oliveira6Maria Alice da Cruz-Höfling7Cláudia Herrera Tambeli8Carlos Amilcar Parada9Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP)Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP)Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP)Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP)Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP)Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP)Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP)Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP)Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP)Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP)Abstract Several recent studies have established the efficacy of photobiomodulation therapy (PBMT) in painful clinical conditions. Diabetic neuropathy (DN) can be related to activating mitogen-activated protein kinases (MAPK), such as p38, in the peripheral nerve. MAPK pathway is activated in response to extracellular stimuli, including interleukins TNF-α and IL-1β. We verified the pain relief potential of PBMT in streptozotocin (STZ)-induced diabetic neuropathic rats and its influence on the MAPK pathway regulation and calcium (Ca2+) dynamics. We then observed that PBMT applied to the L4-L5 dorsal root ganglion (DRG) region reduced the intensity of hyperalgesia, decreased TNF-α and IL-1β levels, and p38-MAPK mRNA expression in DRG of diabetic neuropathic rats. DN induced the activation of phosphorylated p38 (p-38) MAPK co-localized with TRPV1+ neurons; PBMT partially prevented p-38 activation. DN was related to an increase of p38-MAPK expression due to proinflammatory interleukins, and the PBMT (904 nm) treatment counteracted this condition. Also, the sensitization of DRG neurons by the hyperglycemic condition demonstrated during the Ca2+ dynamics was reduced by PBMT, contributing to its anti-hyperalgesic effects.https://doi.org/10.1038/s41598-022-19947-2 |
| spellingShingle | Willians Fernando Vieira Kauê Franco Malange Silviane Fernandes de Magalhães Júlia Borges Paes Lemes Gilson Gonçalves dos Santos Catarine Massucato Nishijima Alexandre Leite Rodrigues de Oliveira Maria Alice da Cruz-Höfling Cláudia Herrera Tambeli Carlos Amilcar Parada Anti-hyperalgesic effects of photobiomodulation therapy (904 nm) on streptozotocin-induced diabetic neuropathy imply MAPK pathway and calcium dynamics modulation Scientific Reports |
| title | Anti-hyperalgesic effects of photobiomodulation therapy (904 nm) on streptozotocin-induced diabetic neuropathy imply MAPK pathway and calcium dynamics modulation |
| title_full | Anti-hyperalgesic effects of photobiomodulation therapy (904 nm) on streptozotocin-induced diabetic neuropathy imply MAPK pathway and calcium dynamics modulation |
| title_fullStr | Anti-hyperalgesic effects of photobiomodulation therapy (904 nm) on streptozotocin-induced diabetic neuropathy imply MAPK pathway and calcium dynamics modulation |
| title_full_unstemmed | Anti-hyperalgesic effects of photobiomodulation therapy (904 nm) on streptozotocin-induced diabetic neuropathy imply MAPK pathway and calcium dynamics modulation |
| title_short | Anti-hyperalgesic effects of photobiomodulation therapy (904 nm) on streptozotocin-induced diabetic neuropathy imply MAPK pathway and calcium dynamics modulation |
| title_sort | anti hyperalgesic effects of photobiomodulation therapy 904 nm on streptozotocin induced diabetic neuropathy imply mapk pathway and calcium dynamics modulation |
| url | https://doi.org/10.1038/s41598-022-19947-2 |
| work_keys_str_mv | AT williansfernandovieira antihyperalgesiceffectsofphotobiomodulationtherapy904nmonstreptozotocininduceddiabeticneuropathyimplymapkpathwayandcalciumdynamicsmodulation AT kauefrancomalange antihyperalgesiceffectsofphotobiomodulationtherapy904nmonstreptozotocininduceddiabeticneuropathyimplymapkpathwayandcalciumdynamicsmodulation AT silvianefernandesdemagalhaes antihyperalgesiceffectsofphotobiomodulationtherapy904nmonstreptozotocininduceddiabeticneuropathyimplymapkpathwayandcalciumdynamicsmodulation AT juliaborgespaeslemes antihyperalgesiceffectsofphotobiomodulationtherapy904nmonstreptozotocininduceddiabeticneuropathyimplymapkpathwayandcalciumdynamicsmodulation AT gilsongoncalvesdossantos antihyperalgesiceffectsofphotobiomodulationtherapy904nmonstreptozotocininduceddiabeticneuropathyimplymapkpathwayandcalciumdynamicsmodulation AT catarinemassucatonishijima antihyperalgesiceffectsofphotobiomodulationtherapy904nmonstreptozotocininduceddiabeticneuropathyimplymapkpathwayandcalciumdynamicsmodulation AT alexandreleiterodriguesdeoliveira antihyperalgesiceffectsofphotobiomodulationtherapy904nmonstreptozotocininduceddiabeticneuropathyimplymapkpathwayandcalciumdynamicsmodulation AT mariaalicedacruzhofling antihyperalgesiceffectsofphotobiomodulationtherapy904nmonstreptozotocininduceddiabeticneuropathyimplymapkpathwayandcalciumdynamicsmodulation AT claudiaherreratambeli antihyperalgesiceffectsofphotobiomodulationtherapy904nmonstreptozotocininduceddiabeticneuropathyimplymapkpathwayandcalciumdynamicsmodulation AT carlosamilcarparada antihyperalgesiceffectsofphotobiomodulationtherapy904nmonstreptozotocininduceddiabeticneuropathyimplymapkpathwayandcalciumdynamicsmodulation |