PCR & Go: A Pre-installed Expression Chassis for Facile Integration of Multi-Gene Biosynthetic Pathways

PCR & Go: A Pre-installed Expression Chassis for Facile Integration of Multi-Gene Biosynthetic Pathways

The introduction of multi-gene metabolic pathways is generally the first step for the construction of microbial cell factories and plays an essential role in metabolic engineering and synthetic biology. Here, we developed a “PCR & Go” system for facile integration and assembly of multi-gene...

Full description

Bibliographic Details
Main Authors: Mingming Qi, Bei Zhang, Lihong Jiang, Saijuan Xu, Chang Dong, Yi-Ling Du, Zhan Zhou, Lei Huang, Zhinan Xu, Jiazhang Lian
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-01-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
multiplex genome integration
CRISPR/Cas9
metabolic engineering
carotenoid biosynthesis
Saccharomyces cerevisiae
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2020.613771/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2020.613771/full

Similar Items