Fusion Tag Design Influences Soluble Recombinant Protein Production in <i>Escherichia coli</i>

Fusion Tag Design Influences Soluble Recombinant Protein Production in <i>Escherichia coli</i>

Fusion protein technologies to facilitate soluble expression, detection, or subsequent affinity purification in <i>Escherichia coli</i> are widely used but may also be associated with negative consequences. Although commonly employed solubility tags have a positive influence on titers, t...

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Bibliographic Details
Main Authors: Christoph Köppl, Nico Lingg, Andreas Fischer, Christina Kröß, Julian Loibl, Wolfgang Buchinger, Rainer Schneider, Alois Jungbauer, Gerald Striedner, Monika Cserjan-Puschmann
Format: Article
Language:English
Published: MDPI AG 2022-07-01
Series:International Journal of Molecular Sciences
Subjects:
bacteriophage-derived tag
fed-batch cultivation
proteolytic cleavage
inclusion body
caspase-2
Online Access:https://www.mdpi.com/1422-0067/23/14/7678

Internet

https://www.mdpi.com/1422-0067/23/14/7678

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