A colony multiplex quantitative PCR-Based 3S3DBC method and variations of it for screening DNA libraries.

A DNA library is a collection of DNA fragments cloned into vectors and stored individually in host cells, and is a valuable resource for molecular cloning, gene physical mapping, and genome sequencing projects. To take the best advantage of a DNA library, a good screening method is needed. After des...

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Bibliographic Details
Main Authors: Yang An, Atsushi Toyoda, Chen Zhao, Asao Fujiyama, Kiyokazu Agata
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0116997&type=printable