Design of novel cyanovirin-N variants by modulation of binding dynamics through distal mutations

Design of novel cyanovirin-N variants by modulation of binding dynamics through distal mutations

We develop integrated co-evolution and dynamic coupling (ICDC) approach to identify, mutate, and assess distal sites to modulate function. We validate the approach first by analyzing the existing mutational fitness data of TEM-1 β-lactamase and show that allosteric positions co-evolved and dynamical...

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Bibliographic Details
Main Authors: I Can Kazan, Prerna Sharma, Mohammad Imtiazur Rahman, Andrey Bobkov, Raimund Fromme, Giovanna Ghirlanda, S Banu Ozkan
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2022-12-01
Series:eLife
Subjects:
protein dynamics
allostery
lectin
glycan
enzyme
molecular mechanism
Online Access:https://elifesciences.org/articles/67474

Internet

https://elifesciences.org/articles/67474

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