Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts

Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts

Abstract Site-specific recombinases such as the Cre-LoxP system are routinely used for genome engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the CRISPR-Cas toolbox and provide the additional benefit of high-efficiency DNA editing without generating toxic DNA dou...

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Sonraí bibleagrafaíochta
Príomhchruthaitheoirí: Charlotte Cautereels, Jolien Smets, Jonas De Saeger, Lloyd Cool, Yanmei Zhu, Anna Zimmermann, Jan Steensels, Anton Gorkovskiy, Thomas B. Jacobs, Kevin J. Verstrepen
Formáid: Alt
Teanga:English
Foilsithe / Cruthaithe: Nature Portfolio 2024-02-01
Sraith:Nature Communications
Rochtain ar líne:https://doi.org/10.1038/s41467-024-44996-8

Ar líne

https://doi.org/10.1038/s41467-024-44996-8

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