Developing CRISPR/Cas9-Mediated Fluorescent Reporter Human Pluripotent Stem-Cell Lines for High-Content Screening

Developing CRISPR/Cas9-Mediated Fluorescent Reporter Human Pluripotent Stem-Cell Lines for High-Content Screening

Application of the CRISPR/Cas9 system to knock in fluorescent proteins to endogenous genes of interest in human pluripotent stem cells (hPSCs) has the potential to facilitate hPSC-based disease modeling, drug screening, and optimization of transplantation therapy. To evaluate the capability of fluor...

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Bibliographic Details
Main Authors: Kinga Vojnits, Mio Nakanishi, Deanna Porras, Yeonjoon Kim, Zhuohang Feng, Diana Golubeva, Mick Bhatia
Format: Article
Language:English
Published: MDPI AG 2022-04-01
Series:Molecules
Subjects:
genome editing
CRISPR/Cas9
OCT4 locus
AAVS1 locus
human pluripotent stem cells
EGFP reporter lines
Online Access:https://www.mdpi.com/1420-3049/27/8/2434

Internet

https://www.mdpi.com/1420-3049/27/8/2434

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