A high-throughput dual system to screen polyphosphate kinase mutants for efficient ATP regeneration in L-theanine biocatalysis

A high-throughput dual system to screen polyphosphate kinase mutants for efficient ATP regeneration in L-theanine biocatalysis

Abstract ATP, an important cofactor, is involved in many biocatalytic reactions that require energy. Polyphosphate kinases (PPK) can provide energy for ATP-consuming reactions due to their cheap and readily available substrate polyphosphate. We determined the catalytic properties of PPK from differe...

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Bibliographic Details
Main Authors: Hui Gao, Mengxuan Li, Qing Wang, Tingting Liu, Xian Zhang, Taowei Yang, Meijuan Xu, Zhiming Rao
Format: Article
Language:English
Published: BMC 2023-08-01
Series:Biotechnology for Biofuels and Bioproducts
Subjects:
Polyphosphate kinase
ATP regeneration
High-throughput screening
Mutation
Online Access:https://doi.org/10.1186/s13068-023-02361-9

Internet

https://doi.org/10.1186/s13068-023-02361-9

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