Simplified plasmid cloning with a universal MCS design and bacterial in vivo assembly
Abstract Background The ability to clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. The recent development of seamless cloning technologies has made significant improvements in plasmid construction, but simple and reliable tools are always desirable...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2021-03-01
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Series: | BMC Biotechnology |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12896-021-00679-6 |