Simplified plasmid cloning with a universal MCS design and bacterial in vivo assembly

Abstract Background The ability to clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. The recent development of seamless cloning technologies has made significant improvements in plasmid construction, but simple and reliable tools are always desirable...

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Bibliographic Details
Main Authors: Fan Chen, Yi-ya Li, Yan-li Yu, Jie Dai, Jin-ling Huang, Jie Lin
Format: Article
Language:English
Published: BMC 2021-03-01
Series:BMC Biotechnology
Subjects:
Online Access:https://doi.org/10.1186/s12896-021-00679-6