Human Complement Receptor Type 1/CD35 Is an Epstein-Barr Virus Receptor
Epstein-Barr virus (EBV) attachment to primary B cells initiates virus entry. Although CD21 is the only known receptor for EBVgp350/220, a recent report documents EBV-infected B cells from a patient genetically deficient in CD21. On normal resting B cells, CD21 forms two membrane complexes: one with...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-02-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124713000302 |
| _version_ | 1818749777074978816 |
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| author | Javier G. Ogembo Lakshmi Kannan Ionita Ghiran Anne Nicholson-Weller Robert W. Finberg George C. Tsokos Joyce D. Fingeroth |
| author_facet | Javier G. Ogembo Lakshmi Kannan Ionita Ghiran Anne Nicholson-Weller Robert W. Finberg George C. Tsokos Joyce D. Fingeroth |
| author_sort | Javier G. Ogembo |
| collection | DOAJ |
| description | Epstein-Barr virus (EBV) attachment to primary B cells initiates virus entry. Although CD21 is the only known receptor for EBVgp350/220, a recent report documents EBV-infected B cells from a patient genetically deficient in CD21. On normal resting B cells, CD21 forms two membrane complexes: one with CD19 and another with CD35. Whereas the CD21/CD19 complex is widely retained on immortalized and B cell tumor lines, the related complement-regulatory protein CD35 is lost. To determine the role(s) of CD35 in initial infection, we transduced a CD21-negative pre-B cell and myeloid leukemia line with CD35, CD21, or both. Cells expressing CD35 alone bound gp350/220 and became latently infected when the fusion receptor HLA II was coexpressed. Temporal, biophysical, and structural characteristics of CD35-mediated infection were distinct from CD21. Identification of CD35 as an EBV receptor uncovers a salient role in primary infection, addresses unsettled questions of virus tropism, and underscores the importance of EBVgp350/220 for vaccine development. |
| first_indexed | 2024-12-18T04:09:10Z |
| format | Article |
| id | doaj.art-11a566a3cf4741ef9163d8e1f2ea3d89 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-18T04:09:10Z |
| publishDate | 2013-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-11a566a3cf4741ef9163d8e1f2ea3d892022-12-21T21:21:31ZengElsevierCell Reports2211-12472013-02-013237138510.1016/j.celrep.2013.01.023Human Complement Receptor Type 1/CD35 Is an Epstein-Barr Virus ReceptorJavier G. Ogembo0Lakshmi Kannan1Ionita Ghiran2Anne Nicholson-Weller3Robert W. Finberg4George C. Tsokos5Joyce D. Fingeroth6Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USADepartment of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USADepartment of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USADepartment of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USADepartment of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USADepartment of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USADepartment of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USAEpstein-Barr virus (EBV) attachment to primary B cells initiates virus entry. Although CD21 is the only known receptor for EBVgp350/220, a recent report documents EBV-infected B cells from a patient genetically deficient in CD21. On normal resting B cells, CD21 forms two membrane complexes: one with CD19 and another with CD35. Whereas the CD21/CD19 complex is widely retained on immortalized and B cell tumor lines, the related complement-regulatory protein CD35 is lost. To determine the role(s) of CD35 in initial infection, we transduced a CD21-negative pre-B cell and myeloid leukemia line with CD35, CD21, or both. Cells expressing CD35 alone bound gp350/220 and became latently infected when the fusion receptor HLA II was coexpressed. Temporal, biophysical, and structural characteristics of CD35-mediated infection were distinct from CD21. Identification of CD35 as an EBV receptor uncovers a salient role in primary infection, addresses unsettled questions of virus tropism, and underscores the importance of EBVgp350/220 for vaccine development.http://www.sciencedirect.com/science/article/pii/S2211124713000302 |
| spellingShingle | Javier G. Ogembo Lakshmi Kannan Ionita Ghiran Anne Nicholson-Weller Robert W. Finberg George C. Tsokos Joyce D. Fingeroth Human Complement Receptor Type 1/CD35 Is an Epstein-Barr Virus Receptor Cell Reports |
| title | Human Complement Receptor Type 1/CD35 Is an Epstein-Barr Virus Receptor |
| title_full | Human Complement Receptor Type 1/CD35 Is an Epstein-Barr Virus Receptor |
| title_fullStr | Human Complement Receptor Type 1/CD35 Is an Epstein-Barr Virus Receptor |
| title_full_unstemmed | Human Complement Receptor Type 1/CD35 Is an Epstein-Barr Virus Receptor |
| title_short | Human Complement Receptor Type 1/CD35 Is an Epstein-Barr Virus Receptor |
| title_sort | human complement receptor type 1 cd35 is an epstein barr virus receptor |
| url | http://www.sciencedirect.com/science/article/pii/S2211124713000302 |
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