An optimized workflow to measure bacterial predation in microplates

Summary: The predatory bacterium Bdellovibrio bacteriovorus invades and proliferates inside other bacteria by non-binary division. Here we describe a fluorescence-based technique for the immediate evaluation of predator density independently of plaque formation, an optimized setup to monitor predati...

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Bibliographic Details
Main Authors: Ophélie Remy, Thomas Lamot, Yoann Santin, Jovana Kaljević, Charles de Pierpont, Géraldine Laloux
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721008108