An optimized workflow to measure bacterial predation in microplates
Summary: The predatory bacterium Bdellovibrio bacteriovorus invades and proliferates inside other bacteria by non-binary division. Here we describe a fluorescence-based technique for the immediate evaluation of predator density independently of plaque formation, an optimized setup to monitor predati...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721008108 |
| _version_ | 1818646504934473728 |
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| author | Ophélie Remy Thomas Lamot Yoann Santin Jovana Kaljević Charles de Pierpont Géraldine Laloux |
| author_facet | Ophélie Remy Thomas Lamot Yoann Santin Jovana Kaljević Charles de Pierpont Géraldine Laloux |
| author_sort | Ophélie Remy |
| collection | DOAJ |
| description | Summary: The predatory bacterium Bdellovibrio bacteriovorus invades and proliferates inside other bacteria by non-binary division. Here we describe a fluorescence-based technique for the immediate evaluation of predator density independently of plaque formation, an optimized setup to monitor predation in microplates, and the CuRveR package to quantify both prey killing and predator proliferation dynamics. This protocol allows to assess the impact of mutations or chemicals on predation. CuRveR also constitutes a user-friendly tool to analyze growth or decay data unrelated to predation.For complete details on the use and execution of this profile, please refer to Kaljević et al., 2021. |
| first_indexed | 2024-12-17T00:47:42Z |
| format | Article |
| id | doaj.art-11a909cde87d43bf872054592c92163e |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T00:47:42Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-11a909cde87d43bf872054592c92163e2022-12-21T22:09:50ZengElsevierSTAR Protocols2666-16672022-03-0131101104An optimized workflow to measure bacterial predation in microplatesOphélie Remy0Thomas Lamot1Yoann Santin2Jovana Kaljević3Charles de Pierpont4Géraldine Laloux5de Duve Institute, UCLouvain, 75 avenue Hippocrate, 1200 Brussels, Belgiumde Duve Institute, UCLouvain, 75 avenue Hippocrate, 1200 Brussels, Belgiumde Duve Institute, UCLouvain, 75 avenue Hippocrate, 1200 Brussels, Belgiumde Duve Institute, UCLouvain, 75 avenue Hippocrate, 1200 Brussels, Belgiumde Duve Institute, UCLouvain, 75 avenue Hippocrate, 1200 Brussels, Belgiumde Duve Institute, UCLouvain, 75 avenue Hippocrate, 1200 Brussels, Belgium; Corresponding authorSummary: The predatory bacterium Bdellovibrio bacteriovorus invades and proliferates inside other bacteria by non-binary division. Here we describe a fluorescence-based technique for the immediate evaluation of predator density independently of plaque formation, an optimized setup to monitor predation in microplates, and the CuRveR package to quantify both prey killing and predator proliferation dynamics. This protocol allows to assess the impact of mutations or chemicals on predation. CuRveR also constitutes a user-friendly tool to analyze growth or decay data unrelated to predation.For complete details on the use and execution of this profile, please refer to Kaljević et al., 2021.http://www.sciencedirect.com/science/article/pii/S2666166721008108Cell BiologyCell-based AssaysMicrobiologyMolecular/Chemical Probes |
| spellingShingle | Ophélie Remy Thomas Lamot Yoann Santin Jovana Kaljević Charles de Pierpont Géraldine Laloux An optimized workflow to measure bacterial predation in microplates STAR Protocols Cell Biology Cell-based Assays Microbiology Molecular/Chemical Probes |
| title | An optimized workflow to measure bacterial predation in microplates |
| title_full | An optimized workflow to measure bacterial predation in microplates |
| title_fullStr | An optimized workflow to measure bacterial predation in microplates |
| title_full_unstemmed | An optimized workflow to measure bacterial predation in microplates |
| title_short | An optimized workflow to measure bacterial predation in microplates |
| title_sort | optimized workflow to measure bacterial predation in microplates |
| topic | Cell Biology Cell-based Assays Microbiology Molecular/Chemical Probes |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721008108 |
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