Modulation of transcription burst amplitude underpins dosage compensation in the Drosophila embryo
Summary: Dosage compensation, the balancing of X-linked gene expression between sexes and to the autosomes, is critical to an organism’s fitness and survival. In Drosophila, dosage compensation involves hypertranscription of the male X chromosome. Here, we use quantitative live imaging and modeling...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723003935 |
| _version_ | 1797846333448519680 |
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| author | Lauren Forbes Beadle Hongpeng Zhou Magnus Rattray Hilary L. Ashe |
| author_facet | Lauren Forbes Beadle Hongpeng Zhou Magnus Rattray Hilary L. Ashe |
| author_sort | Lauren Forbes Beadle |
| collection | DOAJ |
| description | Summary: Dosage compensation, the balancing of X-linked gene expression between sexes and to the autosomes, is critical to an organism’s fitness and survival. In Drosophila, dosage compensation involves hypertranscription of the male X chromosome. Here, we use quantitative live imaging and modeling at single-cell resolution to study X chromosome dosage compensation in Drosophila. We show that the four X chromosome genes studied undergo transcriptional bursting in male and female embryos. Mechanistically, our data reveal that transcriptional upregulation of male X chromosome genes is primarily mediated by a higher RNA polymerase II initiation rate and burst amplitude across the expression domain. In contrast, burst frequency is spatially modulated in nuclei within the expression domain in response to different transcription factor concentrations to tune the transcriptional response. Together, these data show how the local and global regulation of distinct burst parameters can establish the complex transcriptional outputs underpinning developmental patterning. |
| first_indexed | 2024-04-09T17:53:16Z |
| format | Article |
| id | doaj.art-11b317e5b51240e388482d81b8ca4dcd |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-09T17:53:16Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-11b317e5b51240e388482d81b8ca4dcd2023-04-15T05:52:30ZengElsevierCell Reports2211-12472023-04-01424112382Modulation of transcription burst amplitude underpins dosage compensation in the Drosophila embryoLauren Forbes Beadle0Hongpeng Zhou1Magnus Rattray2Hilary L. Ashe3Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UKFaculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UKFaculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK; Corresponding authorFaculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK; Corresponding authorSummary: Dosage compensation, the balancing of X-linked gene expression between sexes and to the autosomes, is critical to an organism’s fitness and survival. In Drosophila, dosage compensation involves hypertranscription of the male X chromosome. Here, we use quantitative live imaging and modeling at single-cell resolution to study X chromosome dosage compensation in Drosophila. We show that the four X chromosome genes studied undergo transcriptional bursting in male and female embryos. Mechanistically, our data reveal that transcriptional upregulation of male X chromosome genes is primarily mediated by a higher RNA polymerase II initiation rate and burst amplitude across the expression domain. In contrast, burst frequency is spatially modulated in nuclei within the expression domain in response to different transcription factor concentrations to tune the transcriptional response. Together, these data show how the local and global regulation of distinct burst parameters can establish the complex transcriptional outputs underpinning developmental patterning.http://www.sciencedirect.com/science/article/pii/S2211124723003935CP: Molecular biology |
| spellingShingle | Lauren Forbes Beadle Hongpeng Zhou Magnus Rattray Hilary L. Ashe Modulation of transcription burst amplitude underpins dosage compensation in the Drosophila embryo Cell Reports CP: Molecular biology |
| title | Modulation of transcription burst amplitude underpins dosage compensation in the Drosophila embryo |
| title_full | Modulation of transcription burst amplitude underpins dosage compensation in the Drosophila embryo |
| title_fullStr | Modulation of transcription burst amplitude underpins dosage compensation in the Drosophila embryo |
| title_full_unstemmed | Modulation of transcription burst amplitude underpins dosage compensation in the Drosophila embryo |
| title_short | Modulation of transcription burst amplitude underpins dosage compensation in the Drosophila embryo |
| title_sort | modulation of transcription burst amplitude underpins dosage compensation in the drosophila embryo |
| topic | CP: Molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723003935 |
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