Next generation multiplexing for digital PCR using a novel melt-based hairpin probe design

Digital PCR (dPCR) is a powerful tool for research and diagnostic applications that require absolute quantification of target molecules or detection of rare events, but the number of nucleic acid targets that can be distinguished within an assay has limited its usefulness. For most dPCR systems, one...

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Bibliographic Details
Main Authors: Rebecca L. Edwards, Johanna E. Takach, Michael J. McAndrew, Jondavid Menteer, Rachel M. Lestz, Douglas Whitman, Lee Ann Baxter-Lowe
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-11-01
Series:Frontiers in Genetics
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2023.1272964/full