In vitro synthesis of 32 translation-factor proteins from a single template reveals impaired ribosomal processivity

Abstract The Protein synthesis Using Recombinant Elements (PURE) system enables transcription and translation of a DNA template from purified components. Therefore, the PURE system-catalyzed generation of RNAs and proteins constituting the PURE system itself represents a major challenge toward a sel...

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Bibliographic Details
Main Authors: Anne Doerr, David Foschepoth, Anthony C. Forster, Christophe Danelon
Format: Article
Language:English
Published: Nature Portfolio 2021-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-020-80827-8