Effect of lactic acid bacteria, yeast, and their mixture on the chemical composition, fermentation quality, and bacterial community of cellulase-treated Pennisetum sinese silage
The present study investigated the effects of Lentilactobacillus buchneri, Saccharomyces cerevisiae, and a mixture of the two on the cellulose degradation and microbial community of cellulase-treated Pennisetum sinese (CTPS) during biological pretreatment. The CTPS was stored without additives (CK)...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1047072/full |
| _version_ | 1811257422483816448 |
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| author | Chaosheng Liao Xiaolong Tang Maoya Li Guangrou Lu Xiaokang Huang Lin Li Mingjie Zhang Yixiao Xie Yixiao Xie Chao Chen Chao Chen Ping Li Ping Li |
| author_facet | Chaosheng Liao Xiaolong Tang Maoya Li Guangrou Lu Xiaokang Huang Lin Li Mingjie Zhang Yixiao Xie Yixiao Xie Chao Chen Chao Chen Ping Li Ping Li |
| author_sort | Chaosheng Liao |
| collection | DOAJ |
| description | The present study investigated the effects of Lentilactobacillus buchneri, Saccharomyces cerevisiae, and a mixture of the two on the cellulose degradation and microbial community of cellulase-treated Pennisetum sinese (CTPS) during biological pretreatment. The CTPS was stored without additives (CK) or with L. buchneri (L), yeast (Y, S. cerevisiae), and their mixture (LY) under anaerobic conditions for 60 days. All inoculants enhanced the anaerobic fermentation of CTPS. In relative to L, inoculations with Y and LY decreased the cellulose level of fermented-CTPS by 8.90 ~ 17.13%. Inoculation with L inhibited the growth of Weissella cibaria during anaerobic storage. However, inoculations with LY increased the relative abundance of the homofermentative bacterium Lactiplantibacillus plantarum by 6.04%. Therefore, inoculating S. cerevisiae reduced the adverse effects of L. buchneri-stimulated fermentation on cellulose degradation by altering the bacterial community during anaerobic storage of P. sinese. This work provides a new insight for the subsequent anaerobic digestion of P. sinese. |
| first_indexed | 2024-04-12T17:57:09Z |
| format | Article |
| id | doaj.art-11f1193d9f7a4694bb84311253fd5667 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T17:57:09Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-11f1193d9f7a4694bb84311253fd56672022-12-22T03:22:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10470721047072Effect of lactic acid bacteria, yeast, and their mixture on the chemical composition, fermentation quality, and bacterial community of cellulase-treated Pennisetum sinese silageChaosheng Liao0Xiaolong Tang1Maoya Li2Guangrou Lu3Xiaokang Huang4Lin Li5Mingjie Zhang6Yixiao Xie7Yixiao Xie8Chao Chen9Chao Chen10Ping Li11Ping Li12College of Animal Science, Guizhou University, Guiyang, ChinaCollege of Animal Science, Guizhou University, Guiyang, ChinaCollege of Animal Science, Guizhou University, Guiyang, ChinaCollege of Animal Science, Guizhou University, Guiyang, ChinaCollege of Animal Science, Guizhou University, Guiyang, ChinaCollege of Animal Science, Guizhou University, Guiyang, ChinaCollege of Animal Science, Guizhou University, Guiyang, ChinaCollege of Animal Science, Guizhou University, Guiyang, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, Guizhou, ChinaCollege of Animal Science, Guizhou University, Guiyang, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, Guizhou, ChinaCollege of Animal Science, Guizhou University, Guiyang, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, Guizhou, ChinaThe present study investigated the effects of Lentilactobacillus buchneri, Saccharomyces cerevisiae, and a mixture of the two on the cellulose degradation and microbial community of cellulase-treated Pennisetum sinese (CTPS) during biological pretreatment. The CTPS was stored without additives (CK) or with L. buchneri (L), yeast (Y, S. cerevisiae), and their mixture (LY) under anaerobic conditions for 60 days. All inoculants enhanced the anaerobic fermentation of CTPS. In relative to L, inoculations with Y and LY decreased the cellulose level of fermented-CTPS by 8.90 ~ 17.13%. Inoculation with L inhibited the growth of Weissella cibaria during anaerobic storage. However, inoculations with LY increased the relative abundance of the homofermentative bacterium Lactiplantibacillus plantarum by 6.04%. Therefore, inoculating S. cerevisiae reduced the adverse effects of L. buchneri-stimulated fermentation on cellulose degradation by altering the bacterial community during anaerobic storage of P. sinese. This work provides a new insight for the subsequent anaerobic digestion of P. sinese.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1047072/fullbiological pretreatmentlactic acid bacteriayeastbacterial communitycellulosePennisetum sinese |
| spellingShingle | Chaosheng Liao Xiaolong Tang Maoya Li Guangrou Lu Xiaokang Huang Lin Li Mingjie Zhang Yixiao Xie Yixiao Xie Chao Chen Chao Chen Ping Li Ping Li Effect of lactic acid bacteria, yeast, and their mixture on the chemical composition, fermentation quality, and bacterial community of cellulase-treated Pennisetum sinese silage Frontiers in Microbiology biological pretreatment lactic acid bacteria yeast bacterial community cellulose Pennisetum sinese |
| title | Effect of lactic acid bacteria, yeast, and their mixture on the chemical composition, fermentation quality, and bacterial community of cellulase-treated Pennisetum sinese silage |
| title_full | Effect of lactic acid bacteria, yeast, and their mixture on the chemical composition, fermentation quality, and bacterial community of cellulase-treated Pennisetum sinese silage |
| title_fullStr | Effect of lactic acid bacteria, yeast, and their mixture on the chemical composition, fermentation quality, and bacterial community of cellulase-treated Pennisetum sinese silage |
| title_full_unstemmed | Effect of lactic acid bacteria, yeast, and their mixture on the chemical composition, fermentation quality, and bacterial community of cellulase-treated Pennisetum sinese silage |
| title_short | Effect of lactic acid bacteria, yeast, and their mixture on the chemical composition, fermentation quality, and bacterial community of cellulase-treated Pennisetum sinese silage |
| title_sort | effect of lactic acid bacteria yeast and their mixture on the chemical composition fermentation quality and bacterial community of cellulase treated pennisetum sinese silage |
| topic | biological pretreatment lactic acid bacteria yeast bacterial community cellulose Pennisetum sinese |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1047072/full |
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