M-TUBE enables large-volume bacterial gene delivery using a high-throughput microfluidic electroporation platform.

Conventional cuvette-based and microfluidics-based electroporation approaches for bacterial gene delivery have distinct advantages, but they are typically limited to relatively small sample volumes, reducing their utility for applications requiring high throughput such as the generation of mutant li...

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Bibliographic Details
Main Authors: Po-Hsun Huang, Sijie Chen, Anthony L Shiver, Rebecca Neal Culver, Kerwyn Casey Huang, Cullen R Buie
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2022-09-01
Series:PLoS Biology
Online Access:https://doi.org/10.1371/journal.pbio.3001727