An Efficient Method for Isolating and Purifying Nuclei from Mice Brain for Single-Molecule Imaging Using High-Speed Atomic Force Microscopy

An Efficient Method for Isolating and Purifying Nuclei from Mice Brain for Single-Molecule Imaging Using High-Speed Atomic Force Microscopy

Nuclear pore complexes (NPCs) on the nuclear membrane surface have a crucial function in controlling the movement of small molecules and macromolecules between the cell nucleus and cytoplasm through their intricate core channel resembling a spiderweb with several layers. Currently, there are few met...

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Bibliographic Details
Main Authors: Yujia Qiu, Elma Sakinatus Sajidah, Sota Kondo, Shinnosuke Narimatsu, Muhammad Isman Sandira, Yoshiki Higashiguchi, Goro Nishide, Azuma Taoka, Masaharu Hazawa, Yuka Inaba, Hiroshi Inoue, Ayami Matsushima, Yuki Okada, Mitsutoshi Nakada, Toshio Ando, Keesiang Lim, Richard W. Wong
Format: Article
Language:English
Published: MDPI AG 2024-02-01
Series:Cells
Subjects:
mouse brain
nuclear pore
high-speed atomic force microscopy (HS-AFM)
strainer
Online Access:https://www.mdpi.com/2073-4409/13/3/279

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https://www.mdpi.com/2073-4409/13/3/279

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