Contribution of autofluorescence from intracellular proteins in multiphoton fluorescence lifetime imaging

Abstract Multiphoton fluorescence lifetime imaging microscopy (MPM-FLIM) is extensively proposed as a non-invasive optical method to study tissue metabolism. The approach is based on recording changes in the fluorescence lifetime attributed to metabolic co-enzymes, of which nicotinamide adenine dinu...

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Bibliographic Details
Main Authors: Monika Malak, Jeemol James, Julie Grantham, Marica B. Ericson
Format: Article
Language:English
Published: Nature Portfolio 2022-10-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-20857-6