Absence of nuclease activity in commonly used oxygen-scavenging systems

Absence of nuclease activity in commonly used oxygen-scavenging systems

Abstract Objective Oxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contaminatio...

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Bibliographic Details
Main Authors: Hailey L. Gahlon, Paul Poudevigne-Durance, David Rueda
Format: Article
Language:English
Published: BMC 2017-11-01
Series:BMC Research Notes
Subjects:
Single-molecule imaging
Oxygen scavenging system
Protocatechuate-3,4-dioxygenase
Protocatechuic acid
Glucose oxidase
Catalase
Online Access:http://link.springer.com/article/10.1186/s13104-017-2929-6

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http://link.springer.com/article/10.1186/s13104-017-2929-6

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