Absence of nuclease activity in commonly used oxygen-scavenging systems
Abstract Objective Oxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contaminatio...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-017-2929-6 |
| _version_ | 1828199575530242048 |
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| author | Hailey L. Gahlon Paul Poudevigne-Durance David Rueda |
| author_facet | Hailey L. Gahlon Paul Poudevigne-Durance David Rueda |
| author_sort | Hailey L. Gahlon |
| collection | DOAJ |
| description | Abstract Objective Oxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contamination in these oxygen scavenging systems. Results Linear and plasmid DNA was incubated with two different oxygen scavenging systems (1) protocatechuic acid (PCA)-protocatechuate-3,4-dioxygenase (PCD) and (2) glucose-coupled glucose oxidase/catalase (GODCAT). No nucleic acid degradation was observed on single and double-stranded linear DNA and plasmid DNA, indicating the absence of nuclease contamination in these oxygen scavenging systems. |
| first_indexed | 2024-04-12T10:57:28Z |
| format | Article |
| id | doaj.art-124eb079dc6b48519d8ce770f25762f9 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-04-12T10:57:28Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-124eb079dc6b48519d8ce770f25762f92022-12-22T03:36:03ZengBMCBMC Research Notes1756-05002017-11-011011410.1186/s13104-017-2929-6Absence of nuclease activity in commonly used oxygen-scavenging systemsHailey L. Gahlon0Paul Poudevigne-Durance1David Rueda2Molecular Virology, Department of Medicine, Imperial College LondonMolecular Virology, Department of Medicine, Imperial College LondonMolecular Virology, Department of Medicine, Imperial College LondonAbstract Objective Oxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contamination in these oxygen scavenging systems. Results Linear and plasmid DNA was incubated with two different oxygen scavenging systems (1) protocatechuic acid (PCA)-protocatechuate-3,4-dioxygenase (PCD) and (2) glucose-coupled glucose oxidase/catalase (GODCAT). No nucleic acid degradation was observed on single and double-stranded linear DNA and plasmid DNA, indicating the absence of nuclease contamination in these oxygen scavenging systems.http://link.springer.com/article/10.1186/s13104-017-2929-6Single-molecule imagingOxygen scavenging systemProtocatechuate-3,4-dioxygenaseProtocatechuic acidGlucose oxidaseCatalase |
| spellingShingle | Hailey L. Gahlon Paul Poudevigne-Durance David Rueda Absence of nuclease activity in commonly used oxygen-scavenging systems BMC Research Notes Single-molecule imaging Oxygen scavenging system Protocatechuate-3,4-dioxygenase Protocatechuic acid Glucose oxidase Catalase |
| title | Absence of nuclease activity in commonly used oxygen-scavenging systems |
| title_full | Absence of nuclease activity in commonly used oxygen-scavenging systems |
| title_fullStr | Absence of nuclease activity in commonly used oxygen-scavenging systems |
| title_full_unstemmed | Absence of nuclease activity in commonly used oxygen-scavenging systems |
| title_short | Absence of nuclease activity in commonly used oxygen-scavenging systems |
| title_sort | absence of nuclease activity in commonly used oxygen scavenging systems |
| topic | Single-molecule imaging Oxygen scavenging system Protocatechuate-3,4-dioxygenase Protocatechuic acid Glucose oxidase Catalase |
| url | http://link.springer.com/article/10.1186/s13104-017-2929-6 |
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