Exopolysaccharides from Pleurotus pulmonarius: Fermentation Optimization, Characterization and Antioxidant Activity
Culture conditions for exopolysaccharide (EPS) production by Pleurotus pulmonarius in submerged culture are optimized. The suggested medium composition was as follows: 60 g/L of xylose, 6 g/L of soy extract, 5 mM of KH2PO4 and 5 mM of MgSO4. Under the optimized culture conditions in a 5-litre stirre...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2013-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/169434 |
| _version_ | 1797431125274001408 |
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| author | Jin-Wen Shen Chao-Wen Shi Chun-Ping Xu |
| author_facet | Jin-Wen Shen Chao-Wen Shi Chun-Ping Xu |
| author_sort | Jin-Wen Shen |
| collection | DOAJ |
| description | Culture conditions for exopolysaccharide (EPS) production by Pleurotus pulmonarius in submerged culture are optimized. The suggested medium composition was as follows: 60 g/L of xylose, 6 g/L of soy extract, 5 mM of KH2PO4 and 5 mM of MgSO4. Under the optimized culture conditions in a 5-litre stirred tank fermentor, the maximum concentration of EPS was 6.36 g/L. Furthermore, the morphological parameters (i.e. average diameter, circularity, roughness and compactness) of the pellets and the broth viscosity are characterized. It has been proven that mycelial morphology and broth viscosity may be the critical parameters affecting the EPS yield. After deproteinization using Sevag method, a group of EPS (designated as fraction) was obtained from the culture filtrates by gel filtration chromatography. FT-IR analysis of the purified EPS revealed prominent characteristic groups corresponding to polyhydric alcohols. GC analysis showed that the purified EPS were mainly composed of galactose and glucose. Furthermore, thermogravimetric analysis indicated that the degradation temperature of the purified EPS was 217 °C. Finally, the antioxidant activity of the EPS fraction was investigated and the relationship with molecular properties was discussed as well. |
| first_indexed | 2024-03-09T09:38:28Z |
| format | Article |
| id | doaj.art-1254689e52db4584bd6db102d13153de |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T09:38:28Z |
| publishDate | 2013-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-1254689e52db4584bd6db102d13153de2023-12-02T01:05:10ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062013-01-01514520527Exopolysaccharides from Pleurotus pulmonarius: Fermentation Optimization, Characterization and Antioxidant ActivityJin-Wen Shen0Chao-Wen Shi1Chun-Ping Xu2College of Life Science, Henan Agriculture University, Zhengzhou, 450002 Henan, PR ChinaCollege of Life Science, Henan Agriculture University, Zhengzhou, 450002 Henan, PR ChinaCollege of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002 Henan, PR ChinaCulture conditions for exopolysaccharide (EPS) production by Pleurotus pulmonarius in submerged culture are optimized. The suggested medium composition was as follows: 60 g/L of xylose, 6 g/L of soy extract, 5 mM of KH2PO4 and 5 mM of MgSO4. Under the optimized culture conditions in a 5-litre stirred tank fermentor, the maximum concentration of EPS was 6.36 g/L. Furthermore, the morphological parameters (i.e. average diameter, circularity, roughness and compactness) of the pellets and the broth viscosity are characterized. It has been proven that mycelial morphology and broth viscosity may be the critical parameters affecting the EPS yield. After deproteinization using Sevag method, a group of EPS (designated as fraction) was obtained from the culture filtrates by gel filtration chromatography. FT-IR analysis of the purified EPS revealed prominent characteristic groups corresponding to polyhydric alcohols. GC analysis showed that the purified EPS were mainly composed of galactose and glucose. Furthermore, thermogravimetric analysis indicated that the degradation temperature of the purified EPS was 217 °C. Finally, the antioxidant activity of the EPS fraction was investigated and the relationship with molecular properties was discussed as well.http://hrcak.srce.hr/file/169434fermentation optimizationPleurotus pulmonariusmorphological and rheological propertiesexopolysaccharide productionthermogravimetric analysisantioxidant activity |
| spellingShingle | Jin-Wen Shen Chao-Wen Shi Chun-Ping Xu Exopolysaccharides from Pleurotus pulmonarius: Fermentation Optimization, Characterization and Antioxidant Activity Food Technology and Biotechnology fermentation optimization Pleurotus pulmonarius morphological and rheological properties exopolysaccharide production thermogravimetric analysis antioxidant activity |
| title | Exopolysaccharides from Pleurotus pulmonarius: Fermentation Optimization, Characterization and Antioxidant Activity |
| title_full | Exopolysaccharides from Pleurotus pulmonarius: Fermentation Optimization, Characterization and Antioxidant Activity |
| title_fullStr | Exopolysaccharides from Pleurotus pulmonarius: Fermentation Optimization, Characterization and Antioxidant Activity |
| title_full_unstemmed | Exopolysaccharides from Pleurotus pulmonarius: Fermentation Optimization, Characterization and Antioxidant Activity |
| title_short | Exopolysaccharides from Pleurotus pulmonarius: Fermentation Optimization, Characterization and Antioxidant Activity |
| title_sort | exopolysaccharides from pleurotus pulmonarius fermentation optimization characterization and antioxidant activity |
| topic | fermentation optimization Pleurotus pulmonarius morphological and rheological properties exopolysaccharide production thermogravimetric analysis antioxidant activity |
| url | http://hrcak.srce.hr/file/169434 |
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