Low-pressure chromatographic separation and UV/Vis spectrophotometric characterization of the native and desialylated human apo-transferrin

Low-pressure chromatographic separation and UV/Vis spectrophotometric characterization of the native and desialylated human apo-transferrin

Low-pressure pH gradient ion exchange separation provides a fast, simple and cost-effective method for preparative purification of native and desialylated apo-transferrin. The method enables easy monitoring of the extent of the desialylation reaction and also the efficient separation and purificatio...

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Bibliographic Details
Main Authors: Tomislav Friganović, Antonela Tomašić, Tino Šeba, Ivan Biruš, Robert Kerep, Valentina Borko, Davor Šakić, Mario Gabričević, Tin Weitner
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:Heliyon
Subjects:
Transferrin
Glycoforms
Sialic acid
Chromatography
UV-Vis spectroscopy
Molar absorbance
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844021021332

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http://www.sciencedirect.com/science/article/pii/S2405844021021332

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