Low-pressure chromatographic separation and UV/Vis spectrophotometric characterization of the native and desialylated human apo-transferrin
Low-pressure pH gradient ion exchange separation provides a fast, simple and cost-effective method for preparative purification of native and desialylated apo-transferrin. The method enables easy monitoring of the extent of the desialylation reaction and also the efficient separation and purificatio...
Main Authors: | Tomislav Friganović, Antonela Tomašić, Tino Šeba, Ivan Biruš, Robert Kerep, Valentina Borko, Davor Šakić, Mario Gabričević, Tin Weitner |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2021-09-01
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Series: | Heliyon |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844021021332 |
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