Deuteration of proteins boosted by cell lysates: high-resolution amide and H<i>α</i> magic-angle-spinning (MAS) NMR without the reprotonation bottleneck
<p>Amide-proton-detected magic-angle-spinning NMR of deuterated proteins has become a main technique in NMR-based structural biology. In standard deuteration protocols that rely on D<span class="inline-formula"><sub>2</sub></span>O-based culture media, non-exc...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2024-04-01
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| Series: | Magnetic Resonance |
| Online Access: | https://mr.copernicus.org/articles/5/33/2024/mr-5-33-2024.pdf |