Tunable translational control using site-specific unnatural amino acid incorporation in Escherichia coli

Translation of target gene transcripts in Escherichia coli harboring UAG amber stop codons can be switched on by the amber-codon-specific incorporation of an exogenously supplied unnatural amino acid, 3-iodo-L-tyrosine. Here, we report that this translational switch can control the translational eff...

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Bibliographic Details
Main Author: Yusuke Kato
Format: Article
Language:English
Published: PeerJ Inc. 2015-04-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/904.pdf