Substantially improving the enantioconvergence of PvEH1, a Phaseolus vulgaris epoxide hydrolase, towards m-chlorostyrene oxide by laboratory evolution

Substantially improving the enantioconvergence of PvEH1, a Phaseolus vulgaris epoxide hydrolase, towards m-chlorostyrene oxide by laboratory evolution

Abstract Background Epoxide hydrolase can regioselectively catalyze the oxirane ring-opening hydrolysis of rac-epoxides producing the corresponding chiral diols. In our laboratory, a gene named pveh1 encoding an EH from Phaseolus vulgaris was cloned. Although the directed modification of PvEH1 was c...

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Bibliographic Details
Main Authors: Xun-Cheng Zong, Chuang Li, Yao-Hui Xu, Die Hu, Bo-Chun Hu, Jia Zang, Min-Chen Wu
Format: Article
Language:English
Published: BMC 2019-11-01
Series:Microbial Cell Factories
Subjects:
Epoxide hydrolase
Enantioconvergence
m-Chlorostyrene oxide
m-Chlorophenyl-1,2-ethanediol
Laboratory evolution
Substrate-binding pocket
Online Access:http://link.springer.com/article/10.1186/s12934-019-1252-4

Internet

http://link.springer.com/article/10.1186/s12934-019-1252-4

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