Protocol for in vivo measurement of basal and insulin-stimulated glucose uptake in mouse tissues
Summary: Here, we present an in vivo protocol for measuring basal and insulin-stimulated glucose uptake in tissues from mice. We describe steps for administering 2-deoxy-D-[1,2–3H]glucose in the presence or absence of insulin via intraperitoneal injections. We then detail tissue collection, tissue p...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2023-06-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723001375 |