Protocol for in vivo measurement of basal and insulin-stimulated glucose uptake in mouse tissues

Summary: Here, we present an in vivo protocol for measuring basal and insulin-stimulated glucose uptake in tissues from mice. We describe steps for administering 2-deoxy-D-[1,2–3H]glucose in the presence or absence of insulin via intraperitoneal injections. We then detail tissue collection, tissue p...

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Bibliographic Details
Main Authors: Meng Zhao, Lianna W. Wat, Katrin J. Svensson
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001375