Real-Time Quantitative PCR: Primer Design, Reference Gene Selection, Calculations and Statistics

Real-time quantitative PCR is a technique that can measure the content of the target nucleic acid sequence of interest in a given sample. It is mainly divided into absolute and relative quantitative methods. The relative quantification is mainly used in gene expressions for functional genomic and tr...

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Bibliographic Details
Main Authors: Zhiwei Chen, Nigel G. Halford, Chenghong Liu
Format: Article
Language:English
Published: MDPI AG 2023-06-01
Series:Metabolites
Subjects:
Online Access:https://www.mdpi.com/2218-1989/13/7/806