Development and validation of PCR-based assays for diagnosis of American cutaneous leishmaniasis and identificatio nof the parasite species

Development and validation of PCR-based assays for diagnosis of American cutaneous leishmaniasis and identificatio nof the parasite species

In this study, PCR assays targeting different Leishmania heat-shock protein 70 gene (hsp70) regions, producing fragments ranging in size from 230-390 bp were developed and evaluated to determine their potential as a tool for the specific molecular diagnosis of cutaneous leishmaniasis (CL). A total o...

Full description

Bibliographic Details
Main Authors: Grazielle Cardoso da Graça, Angela Cristina Volpini, Gustavo Adolfo Sierra Romero, Manoel Paes de Oliveira Neto, Marcia Hueb, Renato Porrozzi, Mariana Côrtes Boité, Elisa Cupolillo
Format: Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 2012-08-01
Series:Memorias do Instituto Oswaldo Cruz
Subjects:
leishmaniasis
molecular diagnosis
species identification
polymerase chain reaction
RFLPs
hsp70
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000500014&lng=en&tlng=en

Internet

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000500014&lng=en&tlng=en

Similar Items