Multiprotease improves amino acid release in vitro, energy, and nutrient utilization in broilers fed diets varying in crude protein levels
ABSTRACT: Low crude protein (CP) diets can reduce nitrogen (N) excretion and costs by increasing N utilization efficiency. Exogenous proteases may further improve protein digestibility in low CP diets. This study first evaluated in vitro the efficacy of a multiprotease on amino acid (AA) release fro...
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Format: | Article |
Language: | English |
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Elsevier
2024-05-01
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Series: | Poultry Science |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124001251 |
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author | Lina M. Peñuela-Sierra Valmiro L. Aragão-Neto Paula Lozano-Cruz Juan Nicolas Mejia-Abaunza Muhammad Ali Joaquin Cabañas-Ojeda Yanlin Yang Maria Camila Alfaro-Wisaquillo Gustavo A. Quintana-Ospina Bindhu Lakshmibai Vasanthakumari Alexandra Wealleans Ye Lao Edgar O. Oviedo-Rondón |
author_facet | Lina M. Peñuela-Sierra Valmiro L. Aragão-Neto Paula Lozano-Cruz Juan Nicolas Mejia-Abaunza Muhammad Ali Joaquin Cabañas-Ojeda Yanlin Yang Maria Camila Alfaro-Wisaquillo Gustavo A. Quintana-Ospina Bindhu Lakshmibai Vasanthakumari Alexandra Wealleans Ye Lao Edgar O. Oviedo-Rondón |
author_sort | Lina M. Peñuela-Sierra |
collection | DOAJ |
description | ABSTRACT: Low crude protein (CP) diets can reduce nitrogen (N) excretion and costs by increasing N utilization efficiency. Exogenous proteases may further improve protein digestibility in low CP diets. This study first evaluated in vitro the efficacy of a multiprotease on amino acid (AA) release from feedstuffs and broiler feed. Later, a broiler study evaluated the effect of feeding corn-soybean meal diets containing 3 CP levels (17, 19, and 21% CP) with supplementation on top of 0 or 2,400 U/kg multiprotease on chicken growth performance, total tract CP, and ileal AA digestibilities, and energy utilization. Ross 708 male chickens were placed in 42 cages and assigned to 6 treatments resulting from a 3 × 2 factorial arrangement. Three isocaloric basal diets were formulated to reduce CP, but all diets maintained digestible Lys:CP in 5.47% and the same ideal protein profile. Data were analyzed in a completely randomized design. On average, the multiprotease increased (P < 0.05) in vitro free AA release by 27.81% in most feedstuffs evaluated compared to the control. For broiler feed, 1,200 U/g multiprotease addition improved (P < 0.001) in vitro free AA release by 18.90%. This multiprotease showed interaction effects (P < 0.05) on chicken FCR, energy, and CP digestibility. As expected, BW at 24 d, BW gain, and FCR (8–24 d) worsened (P < 0.001) as dietary CP reduced from 21 to 17%, and multiprotease addition did not improve (P > 0.05) these parameters. BW gain decreased by 12.9% when N intake was reduced from 49.32 to 38.49 g/bird. Multiprotease supplementation improved (P < 0.01) AMEn by 71 kcal/kg, CP digestibility from 59.45 to 63.51%, ileal AA digestibility, and DM digestibility from 67.08 to 73.49%, but only in the 21% CP diet. No differences in ileal AA digestibility due to CP level (P > 0.05) were detected, except for Cys digestibility (P < 0.01). In conclusion, low CP diets reduced growth performance and improved N utilization but negatively affected energy utilization efficiency. Exogenous multiprotease supplementation improved AME, AMEn, protein, ileal AA, and DM digestibility in the 21% CP diet without significantly affecting growth performance. |
first_indexed | 2024-03-07T18:36:53Z |
format | Article |
id | doaj.art-134fe39dc67647adacfd5263251cbd73 |
institution | Directory Open Access Journal |
issn | 0032-5791 |
language | English |
last_indexed | 2024-03-07T18:36:53Z |
publishDate | 2024-05-01 |
publisher | Elsevier |
record_format | Article |
series | Poultry Science |
spelling | doaj.art-134fe39dc67647adacfd5263251cbd732024-03-02T04:53:27ZengElsevierPoultry Science0032-57912024-05-011035103546Multiprotease improves amino acid release in vitro, energy, and nutrient utilization in broilers fed diets varying in crude protein levelsLina M. Peñuela-Sierra0Valmiro L. Aragão-Neto1Paula Lozano-Cruz2Juan Nicolas Mejia-Abaunza3Muhammad Ali4Joaquin Cabañas-Ojeda5Yanlin Yang6Maria Camila Alfaro-Wisaquillo7Gustavo A. Quintana-Ospina8Bindhu Lakshmibai Vasanthakumari9Alexandra Wealleans10Ye Lao11Edgar O. Oviedo-Rondón12Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USA; College of Veterinary Medicine, Universyty of Tolima, Ibagué, Tolima, 730006299, ColombiaPrestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USAPrestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USA; College of Veterinary Medicine, Universyty of Tolima, Ibagué, Tolima, 730006299, ColombiaPrestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USA; College of Veterinary Medicine, Universyty of Tolima, Ibagué, Tolima, 730006299, ColombiaPrestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USAPrestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USAKemin (China) Technologies Co., Zhuhai, 519040 , ChinaPrestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USAPrestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USAKemin Industries, Des Moines, Iowa 50317, USAKemin Industries, Des Moines, Iowa 50317, USAKemin Industries, Des Moines, Iowa 50317, USAPrestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USA; Corresponding author:ABSTRACT: Low crude protein (CP) diets can reduce nitrogen (N) excretion and costs by increasing N utilization efficiency. Exogenous proteases may further improve protein digestibility in low CP diets. This study first evaluated in vitro the efficacy of a multiprotease on amino acid (AA) release from feedstuffs and broiler feed. Later, a broiler study evaluated the effect of feeding corn-soybean meal diets containing 3 CP levels (17, 19, and 21% CP) with supplementation on top of 0 or 2,400 U/kg multiprotease on chicken growth performance, total tract CP, and ileal AA digestibilities, and energy utilization. Ross 708 male chickens were placed in 42 cages and assigned to 6 treatments resulting from a 3 × 2 factorial arrangement. Three isocaloric basal diets were formulated to reduce CP, but all diets maintained digestible Lys:CP in 5.47% and the same ideal protein profile. Data were analyzed in a completely randomized design. On average, the multiprotease increased (P < 0.05) in vitro free AA release by 27.81% in most feedstuffs evaluated compared to the control. For broiler feed, 1,200 U/g multiprotease addition improved (P < 0.001) in vitro free AA release by 18.90%. This multiprotease showed interaction effects (P < 0.05) on chicken FCR, energy, and CP digestibility. As expected, BW at 24 d, BW gain, and FCR (8–24 d) worsened (P < 0.001) as dietary CP reduced from 21 to 17%, and multiprotease addition did not improve (P > 0.05) these parameters. BW gain decreased by 12.9% when N intake was reduced from 49.32 to 38.49 g/bird. Multiprotease supplementation improved (P < 0.01) AMEn by 71 kcal/kg, CP digestibility from 59.45 to 63.51%, ileal AA digestibility, and DM digestibility from 67.08 to 73.49%, but only in the 21% CP diet. No differences in ileal AA digestibility due to CP level (P > 0.05) were detected, except for Cys digestibility (P < 0.01). In conclusion, low CP diets reduced growth performance and improved N utilization but negatively affected energy utilization efficiency. Exogenous multiprotease supplementation improved AME, AMEn, protein, ileal AA, and DM digestibility in the 21% CP diet without significantly affecting growth performance.http://www.sciencedirect.com/science/article/pii/S0032579124001251low crude proteinbroilerexogenous multiproteaseAA digestibilityAMEn |
spellingShingle | Lina M. Peñuela-Sierra Valmiro L. Aragão-Neto Paula Lozano-Cruz Juan Nicolas Mejia-Abaunza Muhammad Ali Joaquin Cabañas-Ojeda Yanlin Yang Maria Camila Alfaro-Wisaquillo Gustavo A. Quintana-Ospina Bindhu Lakshmibai Vasanthakumari Alexandra Wealleans Ye Lao Edgar O. Oviedo-Rondón Multiprotease improves amino acid release in vitro, energy, and nutrient utilization in broilers fed diets varying in crude protein levels Poultry Science low crude protein broiler exogenous multiprotease AA digestibility AMEn |
title | Multiprotease improves amino acid release in vitro, energy, and nutrient utilization in broilers fed diets varying in crude protein levels |
title_full | Multiprotease improves amino acid release in vitro, energy, and nutrient utilization in broilers fed diets varying in crude protein levels |
title_fullStr | Multiprotease improves amino acid release in vitro, energy, and nutrient utilization in broilers fed diets varying in crude protein levels |
title_full_unstemmed | Multiprotease improves amino acid release in vitro, energy, and nutrient utilization in broilers fed diets varying in crude protein levels |
title_short | Multiprotease improves amino acid release in vitro, energy, and nutrient utilization in broilers fed diets varying in crude protein levels |
title_sort | multiprotease improves amino acid release in vitro energy and nutrient utilization in broilers fed diets varying in crude protein levels |
topic | low crude protein broiler exogenous multiprotease AA digestibility AMEn |
url | http://www.sciencedirect.com/science/article/pii/S0032579124001251 |
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