Listeria monocytogenes folate metabolism is required to generate N-formylmethionine during infection
ABSTRACT Folic acid and its derivatives are required for the synthesis of purines, pyrimidines, and some amino acids. Antifolate antibiotics that target the folic acid metabolism pathway are commonly used for the treatment of listeriosis caused by the intracellular pathogen Listeria monocytogenes (L...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2023-10-01
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| Series: | mBio |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mbio.01385-23 |
| _version_ | 1797619669938470912 |
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| author | Qing Tang Michelle L. Reniere |
| author_facet | Qing Tang Michelle L. Reniere |
| author_sort | Qing Tang |
| collection | DOAJ |
| description | ABSTRACT Folic acid and its derivatives are required for the synthesis of purines, pyrimidines, and some amino acids. Antifolate antibiotics that target the folic acid metabolism pathway are commonly used for the treatment of listeriosis caused by the intracellular pathogen Listeria monocytogenes (Lm). In recent work in mBio, Feng et al. sought to understand the role of folic acid metabolism in Lm virulence (Y. Feng, S. Chang, D. A. Portnoy, 2023, mBio https://doi.org/10.1128/mbio.01074-23). The authors discovered that N-formylmethionine, an amino acid utilized by bacteria to initiate protein synthesis, is crucial for Lm intracellular growth and pathogenesis. Surprisingly, purines and thymidine were found to be dispensable for Lm infection. Together these results demonstrate that while Lm can obtain many essential nutrients from the host cytosol, including purines and most amino acids, it requires N-formylmethionine biosynthesis to properly regulate translation initiation during infection. |
| first_indexed | 2024-03-11T08:30:03Z |
| format | Article |
| id | doaj.art-1353f6aabc3c47a483d6a86bb44a9b3a |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-03-11T08:30:03Z |
| publishDate | 2023-10-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-1353f6aabc3c47a483d6a86bb44a9b3a2023-11-16T21:48:01ZengAmerican Society for MicrobiologymBio2150-75112023-10-0114510.1128/mbio.01385-23Listeria monocytogenes folate metabolism is required to generate N-formylmethionine during infectionQing Tang0Michelle L. Reniere1Department of Microbiology, School of Medicine, University of Washington , Seattle, Washington, USADepartment of Microbiology, School of Medicine, University of Washington , Seattle, Washington, USAABSTRACT Folic acid and its derivatives are required for the synthesis of purines, pyrimidines, and some amino acids. Antifolate antibiotics that target the folic acid metabolism pathway are commonly used for the treatment of listeriosis caused by the intracellular pathogen Listeria monocytogenes (Lm). In recent work in mBio, Feng et al. sought to understand the role of folic acid metabolism in Lm virulence (Y. Feng, S. Chang, D. A. Portnoy, 2023, mBio https://doi.org/10.1128/mbio.01074-23). The authors discovered that N-formylmethionine, an amino acid utilized by bacteria to initiate protein synthesis, is crucial for Lm intracellular growth and pathogenesis. Surprisingly, purines and thymidine were found to be dispensable for Lm infection. Together these results demonstrate that while Lm can obtain many essential nutrients from the host cytosol, including purines and most amino acids, it requires N-formylmethionine biosynthesis to properly regulate translation initiation during infection.https://journals.asm.org/doi/10.1128/mbio.01385-23virulencetranslational controlfolate metabolismListeria monocytogenes |
| spellingShingle | Qing Tang Michelle L. Reniere Listeria monocytogenes folate metabolism is required to generate N-formylmethionine during infection mBio virulence translational control folate metabolism Listeria monocytogenes |
| title | Listeria monocytogenes folate metabolism is required to generate N-formylmethionine during infection |
| title_full | Listeria monocytogenes folate metabolism is required to generate N-formylmethionine during infection |
| title_fullStr | Listeria monocytogenes folate metabolism is required to generate N-formylmethionine during infection |
| title_full_unstemmed | Listeria monocytogenes folate metabolism is required to generate N-formylmethionine during infection |
| title_short | Listeria monocytogenes folate metabolism is required to generate N-formylmethionine during infection |
| title_sort | listeria monocytogenes folate metabolism is required to generate n formylmethionine during infection |
| topic | virulence translational control folate metabolism Listeria monocytogenes |
| url | https://journals.asm.org/doi/10.1128/mbio.01385-23 |
| work_keys_str_mv | AT qingtang listeriamonocytogenesfolatemetabolismisrequiredtogeneratenformylmethionineduringinfection AT michellelreniere listeriamonocytogenesfolatemetabolismisrequiredtogeneratenformylmethionineduringinfection |