Variations in the Sporulation Efficiency of Pathogenic Freshwater Oomycetes in Relation to the Physico-Chemical Properties of Natural Waters
Oomycete pathogens in freshwaters, such as <i>Saprolegnia parasitica</i> and <i>Aphanomyces astaci</i>, are responsible for fish/crayfish population declines in the wild and disease outbreaks in aquaculture. Although the formation of infectious zoospores in the laboratory can...
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MDPI AG
2022-02-01
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| author | Dora Pavić Dorotea Grbin Marija Gregov Josip Ćurko Tomislav Vladušić Lidija Šver Anđela Miljanović Ana Bielen |
| author_facet | Dora Pavić Dorotea Grbin Marija Gregov Josip Ćurko Tomislav Vladušić Lidija Šver Anđela Miljanović Ana Bielen |
| author_sort | Dora Pavić |
| collection | DOAJ |
| description | Oomycete pathogens in freshwaters, such as <i>Saprolegnia parasitica</i> and <i>Aphanomyces astaci</i>, are responsible for fish/crayfish population declines in the wild and disease outbreaks in aquaculture. Although the formation of infectious zoospores in the laboratory can be triggered by washing their mycelium with natural water samples, the physico-chemical properties of the water that might promote sporulation are still unexplored. We washed the mycelia of <i>A. astaci</i> and <i>S. parasitica</i> with a range of natural water samples and observed differences in sporulation efficiency. The results of Partial Least Squares Regression (PLS-R) multivariate analysis showed that SAC (spectral absorption coefficient measured at 254 nm), DOC (dissolved organic carbon), ammonium-N and fluoride had the strongest positive effect on sporulation of <i>S. parasitica</i>, while sporulation of <i>A. astaci</i> was not significantly correlated with any of the analyzed parameters. In agreement with this, the addition of environmentally relevant concentrations of humic acid, an important contributor to SAC and DOC, to the water induced sporulation of <i>S. parasitica</i> but not of <i>A. astaci</i>. Overall, our results point to the differences in ecological requirements of these pathogens, but also present a starting point for optimizing laboratory protocols for the induction of sporulation. |
| first_indexed | 2024-03-09T13:16:08Z |
| format | Article |
| id | doaj.art-136409243f7848a88ba150e50ed661ca |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:16:08Z |
| publishDate | 2022-02-01 |
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| series | Microorganisms |
| spelling | doaj.art-136409243f7848a88ba150e50ed661ca2023-11-30T21:35:26ZengMDPI AGMicroorganisms2076-26072022-02-0110352010.3390/microorganisms10030520Variations in the Sporulation Efficiency of Pathogenic Freshwater Oomycetes in Relation to the Physico-Chemical Properties of Natural WatersDora Pavić0Dorotea Grbin1Marija Gregov2Josip Ćurko3Tomislav Vladušić4Lidija Šver5Anđela Miljanović6Ana Bielen7Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaOomycete pathogens in freshwaters, such as <i>Saprolegnia parasitica</i> and <i>Aphanomyces astaci</i>, are responsible for fish/crayfish population declines in the wild and disease outbreaks in aquaculture. Although the formation of infectious zoospores in the laboratory can be triggered by washing their mycelium with natural water samples, the physico-chemical properties of the water that might promote sporulation are still unexplored. We washed the mycelia of <i>A. astaci</i> and <i>S. parasitica</i> with a range of natural water samples and observed differences in sporulation efficiency. The results of Partial Least Squares Regression (PLS-R) multivariate analysis showed that SAC (spectral absorption coefficient measured at 254 nm), DOC (dissolved organic carbon), ammonium-N and fluoride had the strongest positive effect on sporulation of <i>S. parasitica</i>, while sporulation of <i>A. astaci</i> was not significantly correlated with any of the analyzed parameters. In agreement with this, the addition of environmentally relevant concentrations of humic acid, an important contributor to SAC and DOC, to the water induced sporulation of <i>S. parasitica</i> but not of <i>A. astaci</i>. Overall, our results point to the differences in ecological requirements of these pathogens, but also present a starting point for optimizing laboratory protocols for the induction of sporulation.https://www.mdpi.com/2076-2607/10/3/520<i>Aphanomyces astaci</i>crayfish plaguedissolved organic carbon (DOC)humic acid (HA)<i>Saprolegnia parasitica</i>saprolegniosis |
| spellingShingle | Dora Pavić Dorotea Grbin Marija Gregov Josip Ćurko Tomislav Vladušić Lidija Šver Anđela Miljanović Ana Bielen Variations in the Sporulation Efficiency of Pathogenic Freshwater Oomycetes in Relation to the Physico-Chemical Properties of Natural Waters Microorganisms <i>Aphanomyces astaci</i> crayfish plague dissolved organic carbon (DOC) humic acid (HA) <i>Saprolegnia parasitica</i> saprolegniosis |
| title | Variations in the Sporulation Efficiency of Pathogenic Freshwater Oomycetes in Relation to the Physico-Chemical Properties of Natural Waters |
| title_full | Variations in the Sporulation Efficiency of Pathogenic Freshwater Oomycetes in Relation to the Physico-Chemical Properties of Natural Waters |
| title_fullStr | Variations in the Sporulation Efficiency of Pathogenic Freshwater Oomycetes in Relation to the Physico-Chemical Properties of Natural Waters |
| title_full_unstemmed | Variations in the Sporulation Efficiency of Pathogenic Freshwater Oomycetes in Relation to the Physico-Chemical Properties of Natural Waters |
| title_short | Variations in the Sporulation Efficiency of Pathogenic Freshwater Oomycetes in Relation to the Physico-Chemical Properties of Natural Waters |
| title_sort | variations in the sporulation efficiency of pathogenic freshwater oomycetes in relation to the physico chemical properties of natural waters |
| topic | <i>Aphanomyces astaci</i> crayfish plague dissolved organic carbon (DOC) humic acid (HA) <i>Saprolegnia parasitica</i> saprolegniosis |
| url | https://www.mdpi.com/2076-2607/10/3/520 |
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