Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords

Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords

Summary: Precisely measuring the number and somatic volume of neurons in the central nervous system at single-cell resolution is technically challenging. Here, we combine multiple techniques to address this challenge in optically cleared mouse spinal cords. We describe in vivo neuron labeling approa...

Full description

Bibliographic Details
Main Authors: Hao Yu, Qiang Li, Alfredo Sandoval, Jr., Holly C. Gibbs, Amber English, Tiffany Dunn, John Moth, Hajira Elahi, Bo Chen
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Microscopy
Neuroscience
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722006396

Internet

http://www.sciencedirect.com/science/article/pii/S2666166722006396

Similar Items