Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords
Summary: Precisely measuring the number and somatic volume of neurons in the central nervous system at single-cell resolution is technically challenging. Here, we combine multiple techniques to address this challenge in optically cleared mouse spinal cords. We describe in vivo neuron labeling approa...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722006396 |
| _version_ | 1811199589768757248 |
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| author | Hao Yu Qiang Li Alfredo Sandoval, Jr. Holly C. Gibbs Amber English Tiffany Dunn John Moth Hajira Elahi Bo Chen |
| author_facet | Hao Yu Qiang Li Alfredo Sandoval, Jr. Holly C. Gibbs Amber English Tiffany Dunn John Moth Hajira Elahi Bo Chen |
| author_sort | Hao Yu |
| collection | DOAJ |
| description | Summary: Precisely measuring the number and somatic volume of neurons in the central nervous system at single-cell resolution is technically challenging. Here, we combine multiple techniques to address this challenge in optically cleared mouse spinal cords. We describe in vivo neuron labeling approaches, tissue-clearing technology, light sheet fluorescence microscopy, and machine learning-guided imaging analysis. This combination provides a precise determination of the cell number and somatic volume of any neuron population in the spinal cords. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-12T01:51:09Z |
| format | Article |
| id | doaj.art-136a035447f9461c89fbd00020d4325f |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-12T01:51:09Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-136a035447f9461c89fbd00020d4325f2022-12-22T03:52:56ZengElsevierSTAR Protocols2666-16672022-12-0134101759Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cordsHao Yu0Qiang Li1Alfredo Sandoval, Jr.2Holly C. Gibbs3Amber English4Tiffany Dunn5John Moth6Hajira Elahi7Bo Chen8Department of Neurobiology, University of Texas Medical Branch, Galveston, TX 77550, USA; Corresponding authorDepartment of Neurobiology, University of Texas Medical Branch, Galveston, TX 77550, USADepartment of Neurobiology, University of Texas Medical Branch, Galveston, TX 77550, USAMicroscopy and Imaging Center, Texas A&M University, College Station, TX 77843, USA; Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USAApplication Support Specialist, Bitplane Inc., 300 Baker Avenue Extension No. 150, Concord, MA 01742, USADepartment of Neurobiology, University of Texas Medical Branch, Galveston, TX 77550, USADepartment of Neurobiology, University of Texas Medical Branch, Galveston, TX 77550, USASchool of Behavioral and Brain Sciences, University of Texas at Dallas, Richardson, TX 75080, USADepartment of Neurobiology, University of Texas Medical Branch, Galveston, TX 77550, USA; Corresponding authorSummary: Precisely measuring the number and somatic volume of neurons in the central nervous system at single-cell resolution is technically challenging. Here, we combine multiple techniques to address this challenge in optically cleared mouse spinal cords. We describe in vivo neuron labeling approaches, tissue-clearing technology, light sheet fluorescence microscopy, and machine learning-guided imaging analysis. This combination provides a precise determination of the cell number and somatic volume of any neuron population in the spinal cords. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722006396Cell BiologyMicroscopyNeuroscienceMolecular/Chemical Probes |
| spellingShingle | Hao Yu Qiang Li Alfredo Sandoval, Jr. Holly C. Gibbs Amber English Tiffany Dunn John Moth Hajira Elahi Bo Chen Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords STAR Protocols Cell Biology Microscopy Neuroscience Molecular/Chemical Probes |
| title | Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords |
| title_full | Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords |
| title_fullStr | Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords |
| title_full_unstemmed | Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords |
| title_short | Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords |
| title_sort | pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords |
| topic | Cell Biology Microscopy Neuroscience Molecular/Chemical Probes |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722006396 |
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