A tool to automatically design multiplex PCR primer pairs for specific targets using diverse templates

Abstract Multiplex PCR is an increasingly popular method for identifying species, investigating environmental diversity, and conducting phylogenetic analysis. The complexity and increasing availability of diverse templates necessitate a highly automated approach to design degenerate primer pairs for...

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Bibliografiske detaljer
Main Authors: Lin Yang, Feng Ding, Qiang Lin, Junhua Xie, Wei Fan, Fangyin Dai, Peng Cui, Wanfei Liu
Format: Article
Sprog:English
Udgivet: Nature Portfolio 2023-09-01
Serier:Scientific Reports
Online adgang:https://doi.org/10.1038/s41598-023-43825-0