A Bench-Top Approach for Isolation of Single Antibody Producing Chinese Hamster Ovary (CHO) Cells Using a Microwell-Based Microfluidic Device

A Bench-Top Approach for Isolation of Single Antibody Producing Chinese Hamster Ovary (CHO) Cells Using a Microwell-Based Microfluidic Device

Genetically-modified monoclonal cell lines are currently used for monoclonal antibody (mAbs) production and drug development. The isolation of single transformed cells is the main hindrance in the generation of monoclonal lines. Although the conventional limiting dilution method is time-consuming, l...

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Bibliographic Details
Main Authors: Salma Fuadiyah, Kittipat Chotchindakun, Rungrueang Phatthanakun, Panwong Kuntanawat, Montarop Yamabhai
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:Micromachines
Subjects:
monoclonal cell lines
cell line generation
single-cell cloning
limiting dilution
microfluidic device
Online Access:https://www.mdpi.com/2072-666X/13/11/1939

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https://www.mdpi.com/2072-666X/13/11/1939

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