Conditional reverse tet-transactivator mouse strains for the efficient induction of TRE-regulated transgenes in mice.
Tetracycline or doxycycline (dox)-regulated control of genetic elements allows inducible, reversible and tissue specific regulation of gene expression in mice. This approach provides a means to investigate protein function in specific cell lineages and at defined periods of development and disease....
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3990578?pdf=render |
| _version_ | 1829499486288740352 |
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| author | Lukas E Dow Zeina Nasr Michael Saborowski Saya H Ebbesen Eusebio Manchado Nilgun Tasdemir Teresa Lee Jerry Pelletier Scott W Lowe |
| author_facet | Lukas E Dow Zeina Nasr Michael Saborowski Saya H Ebbesen Eusebio Manchado Nilgun Tasdemir Teresa Lee Jerry Pelletier Scott W Lowe |
| author_sort | Lukas E Dow |
| collection | DOAJ |
| description | Tetracycline or doxycycline (dox)-regulated control of genetic elements allows inducible, reversible and tissue specific regulation of gene expression in mice. This approach provides a means to investigate protein function in specific cell lineages and at defined periods of development and disease. Efficient and stable regulation of cDNAs or non-coding elements (e.g. shRNAs) downstream of the tetracycline-regulated element (TRE) requires the robust expression of a tet-transactivator protein, commonly the reverse tet-transactivator, rtTA. Most rtTA strains rely on tissue specific promoters that often do not provide sufficient rtTA levels for optimal inducible expression. Here we describe the generation of two mouse strains that enable Cre-dependent, robust expression of rtTA3, providing tissue-restricted and consistent induction of TRE-controlled transgenes. We show that these transgenic strains can be effectively combined with established mouse models of disease, including both Cre/LoxP-based approaches and non Cre-dependent disease models. The integration of these new tools with established mouse models promises the development of more flexible genetic systems to uncover the mechanisms of development and disease pathogenesis. |
| first_indexed | 2024-12-16T08:35:34Z |
| format | Article |
| id | doaj.art-13acac9bb382401abf4d9568ca84c4b5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T08:35:34Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-13acac9bb382401abf4d9568ca84c4b52022-12-21T22:37:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9523610.1371/journal.pone.0095236Conditional reverse tet-transactivator mouse strains for the efficient induction of TRE-regulated transgenes in mice.Lukas E DowZeina NasrMichael SaborowskiSaya H EbbesenEusebio ManchadoNilgun TasdemirTeresa LeeJerry PelletierScott W LoweTetracycline or doxycycline (dox)-regulated control of genetic elements allows inducible, reversible and tissue specific regulation of gene expression in mice. This approach provides a means to investigate protein function in specific cell lineages and at defined periods of development and disease. Efficient and stable regulation of cDNAs or non-coding elements (e.g. shRNAs) downstream of the tetracycline-regulated element (TRE) requires the robust expression of a tet-transactivator protein, commonly the reverse tet-transactivator, rtTA. Most rtTA strains rely on tissue specific promoters that often do not provide sufficient rtTA levels for optimal inducible expression. Here we describe the generation of two mouse strains that enable Cre-dependent, robust expression of rtTA3, providing tissue-restricted and consistent induction of TRE-controlled transgenes. We show that these transgenic strains can be effectively combined with established mouse models of disease, including both Cre/LoxP-based approaches and non Cre-dependent disease models. The integration of these new tools with established mouse models promises the development of more flexible genetic systems to uncover the mechanisms of development and disease pathogenesis.http://europepmc.org/articles/PMC3990578?pdf=render |
| spellingShingle | Lukas E Dow Zeina Nasr Michael Saborowski Saya H Ebbesen Eusebio Manchado Nilgun Tasdemir Teresa Lee Jerry Pelletier Scott W Lowe Conditional reverse tet-transactivator mouse strains for the efficient induction of TRE-regulated transgenes in mice. PLoS ONE |
| title | Conditional reverse tet-transactivator mouse strains for the efficient induction of TRE-regulated transgenes in mice. |
| title_full | Conditional reverse tet-transactivator mouse strains for the efficient induction of TRE-regulated transgenes in mice. |
| title_fullStr | Conditional reverse tet-transactivator mouse strains for the efficient induction of TRE-regulated transgenes in mice. |
| title_full_unstemmed | Conditional reverse tet-transactivator mouse strains for the efficient induction of TRE-regulated transgenes in mice. |
| title_short | Conditional reverse tet-transactivator mouse strains for the efficient induction of TRE-regulated transgenes in mice. |
| title_sort | conditional reverse tet transactivator mouse strains for the efficient induction of tre regulated transgenes in mice |
| url | http://europepmc.org/articles/PMC3990578?pdf=render |
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