Optimisation of a 96-well electroporation assay for postnatal rat CNS neurons suitable for cost-effective medium-throughput screening of genes that promote neurite outgrowth

Following an injury, central nervous system (CNS) neurons show a very limited regenerative response which results in their failure to successfully form functional connections with their original target. This is due in part to the reduced intrinsic growth state of CNS neurons, which is characterised...

Full description

Bibliographic Details
Main Authors: Thomas eHutson, William J Buchser, John L Bixby, Vance P Lemmon, Lawrence DF Moon
Format: Article
Language:English
Published: Frontiers Media S.A. 2011-12-01
Series:Frontiers in Molecular Neuroscience
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fnmol.2011.00055/full