Effect of Different Extraction Methods on Physicochemical Characteristics and Antioxidant Activity of C-Phycocyanin from Dry Biomass of <i>Arthrospira platensis</i>
The effect of four different extraction methods on physicochemical characteristics and functionalities of chloro-phycocyanin (CP) was investigated. Swelling (S-CP), freezing and thawing (4FT-CP), ultrasonication with freezing and thawing (4FT+U-CP), and the high-pressure cell disruption (HPCD-CP) pr...
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MDPI AG
2022-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/9/1296 |
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| author | Qian Chen Shuhui Li Hua Xiong Qiang Zhao |
| author_facet | Qian Chen Shuhui Li Hua Xiong Qiang Zhao |
| author_sort | Qian Chen |
| collection | DOAJ |
| description | The effect of four different extraction methods on physicochemical characteristics and functionalities of chloro-phycocyanin (CP) was investigated. Swelling (S-CP), freezing and thawing (4FT-CP), ultrasonication with freezing and thawing (4FT+U-CP), and the high-pressure cell disruption (HPCD-CP) process affected CP differently, thus resulting in different levels of solubility, DPPH scavenging activity, ABTS scavenging activity, and reducing power. Among the four CPs, HPCD-CP had the highest CP content (15.3%), purity (1.66 ± 0.16), and ∆E value but the lowest ∆b value. The ζ potential of HPCD-CP (−38.8 mV) was the highest, but the average particle size of 4FT+U-CP (719.1 nm) was the highest. UV-Vis absorption spectra and fluorescence spectra illustrated that high-pressure cell disruption-assisted extraction had more profound impacts on the microenvironment of tetrapyrrole chromophores, the environment of aromatic amino acids, and the phycocyanobilin of CP. Furthermore, HPCD-CP and 4FT-CP showed higher solubility and antioxidant activities than S-CP, especially 4FT+U-CP. The results obtained in this study demonstrate that HPCD technology could obtain a food-grade C-phycocyanin product with higher CP concentration, purity, solubility, and antioxidant activity. |
| first_indexed | 2024-03-10T04:09:51Z |
| format | Article |
| id | doaj.art-13e4e03a48ae47ec97d6664cfa3c46a8 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T04:09:51Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-13e4e03a48ae47ec97d6664cfa3c46a82023-11-23T08:13:27ZengMDPI AGFoods2304-81582022-04-01119129610.3390/foods11091296Effect of Different Extraction Methods on Physicochemical Characteristics and Antioxidant Activity of C-Phycocyanin from Dry Biomass of <i>Arthrospira platensis</i>Qian Chen0Shuhui Li1Hua Xiong2Qiang Zhao3State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaJiangxi Academy of Agricultural Sciences, Nanchang 330200, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaThe effect of four different extraction methods on physicochemical characteristics and functionalities of chloro-phycocyanin (CP) was investigated. Swelling (S-CP), freezing and thawing (4FT-CP), ultrasonication with freezing and thawing (4FT+U-CP), and the high-pressure cell disruption (HPCD-CP) process affected CP differently, thus resulting in different levels of solubility, DPPH scavenging activity, ABTS scavenging activity, and reducing power. Among the four CPs, HPCD-CP had the highest CP content (15.3%), purity (1.66 ± 0.16), and ∆E value but the lowest ∆b value. The ζ potential of HPCD-CP (−38.8 mV) was the highest, but the average particle size of 4FT+U-CP (719.1 nm) was the highest. UV-Vis absorption spectra and fluorescence spectra illustrated that high-pressure cell disruption-assisted extraction had more profound impacts on the microenvironment of tetrapyrrole chromophores, the environment of aromatic amino acids, and the phycocyanobilin of CP. Furthermore, HPCD-CP and 4FT-CP showed higher solubility and antioxidant activities than S-CP, especially 4FT+U-CP. The results obtained in this study demonstrate that HPCD technology could obtain a food-grade C-phycocyanin product with higher CP concentration, purity, solubility, and antioxidant activity.https://www.mdpi.com/2304-8158/11/9/1296C-phycocyaninhigh-pressure cell disruptionantioxidant activityextraction method<i>Arthrospira platensis</i> |
| spellingShingle | Qian Chen Shuhui Li Hua Xiong Qiang Zhao Effect of Different Extraction Methods on Physicochemical Characteristics and Antioxidant Activity of C-Phycocyanin from Dry Biomass of <i>Arthrospira platensis</i> Foods C-phycocyanin high-pressure cell disruption antioxidant activity extraction method <i>Arthrospira platensis</i> |
| title | Effect of Different Extraction Methods on Physicochemical Characteristics and Antioxidant Activity of C-Phycocyanin from Dry Biomass of <i>Arthrospira platensis</i> |
| title_full | Effect of Different Extraction Methods on Physicochemical Characteristics and Antioxidant Activity of C-Phycocyanin from Dry Biomass of <i>Arthrospira platensis</i> |
| title_fullStr | Effect of Different Extraction Methods on Physicochemical Characteristics and Antioxidant Activity of C-Phycocyanin from Dry Biomass of <i>Arthrospira platensis</i> |
| title_full_unstemmed | Effect of Different Extraction Methods on Physicochemical Characteristics and Antioxidant Activity of C-Phycocyanin from Dry Biomass of <i>Arthrospira platensis</i> |
| title_short | Effect of Different Extraction Methods on Physicochemical Characteristics and Antioxidant Activity of C-Phycocyanin from Dry Biomass of <i>Arthrospira platensis</i> |
| title_sort | effect of different extraction methods on physicochemical characteristics and antioxidant activity of c phycocyanin from dry biomass of i arthrospira platensis i |
| topic | C-phycocyanin high-pressure cell disruption antioxidant activity extraction method <i>Arthrospira platensis</i> |
| url | https://www.mdpi.com/2304-8158/11/9/1296 |
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