Improving the Thermostability of Serine Protease PB92 from <i>Bacillus alcalophilus</i> via Site-Directed Mutagenesis Based on Semi-Rational Design

Proteases have been widely employed in many industrial processes. In this work, we aimed to improve the thermostability of the serine protease PB92 from <i>Bacillus alcalophilus</i> to meet the high-temperature requirements of biotechnological treatments. Eight mutation sites (N18, S97-S...

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Bibliographic Details
Main Authors: Huabiao Miao, Xia Xiang, Nanyu Han, Qian Wu, Zunxi Huang
Format: Article
Language:English
Published: MDPI AG 2023-08-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/12/16/3081