Generation of Mouse Model of Hemophilia A by Introducing Novel Mutations, Using CRISPR/Nickase Gene Targeting System
Developing mouse models of hemophilia A has been shown to facilitate in vivo studies to explore the probablemechanism(s) underlying the disease and to examine the efficiency of the relevant potential therapeutics. This studyaimed to knockout (KO) the coagulation factor viii (fviii) gene in NMRI mice...
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| Format: | Article |
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Royan Institute (ACECR), Tehran
2023-09-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_705505_27aeb300fbfc88bb89c5dce7a7ca5d3a.pdf |
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| author | Mehdi Shamsara Abbas Jamshidizad Aidin Rahim-Tayefeh Maliheh Davari Ali Rajabi Zangi Fatemeh Masoumi Alireza Zomorodipour |
| author_facet | Mehdi Shamsara Abbas Jamshidizad Aidin Rahim-Tayefeh Maliheh Davari Ali Rajabi Zangi Fatemeh Masoumi Alireza Zomorodipour |
| author_sort | Mehdi Shamsara |
| collection | DOAJ |
| description | Developing mouse models of hemophilia A has been shown to facilitate in vivo studies to explore the probablemechanism(s) underlying the disease and to examine the efficiency of the relevant potential therapeutics. This studyaimed to knockout (KO) the coagulation factor viii (fviii) gene in NMRI mice, using CRISPR/Cas9 (D10A/nickase) system,to generate a mouse model of hemophilia A. Two single guide RNAs (sgRNAs), designed from two distinct regions onNMRI mouse FVIII (mFVIII) exon 3, were designed and inserted in the pX335 vector, expressing both sgRNAs andnickase. The recombinant construct was delivered into mouse zygotes and implanted into the pseudopregnant femalemice’s uterus. Mutant mice were identified by genotyping, genomic sequencing, and mFVIII activity assessment. Twoseparate lines of hemophilia A were obtained through interbreeding the offspring of the female mice receiving potentialCRISPR-Cas9-edited zygotes. Genomic DNA analysis revealed disruptions of the mfviii gene reading frame througha 22-bp deletion and a 23-bp insertion in two separate founder mice. The founder mice showed all the clinical signsof hemophilia A including; excessive bleeding after injuries, and spontaneous bleeding in joints and other organs.Coagulation test data showed that mFVIII coagulation activity was significantly diminished in the mFVIII knockout(FVIIIKO) mice compared to normal mice. The CRISPR/nickase system was successfully applied to generate mouselines with the knockout fviii gene. The two novel FVIIIKO mice demonstrated all clinical symptoms of hemophilia A, whichcould be successfully inherited. Therefore, both of the developed FVIIIKO mouse lines are eligible for being consideredas proper mouse models of hemophilia A for in vivo therapeutic studies. |
| first_indexed | 2024-03-11T23:57:49Z |
| format | Article |
| id | doaj.art-140403dc53584f8aaf65e3f4f1dc6ff1 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-03-11T23:57:49Z |
| publishDate | 2023-09-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-140403dc53584f8aaf65e3f4f1dc6ff12023-09-18T06:58:14ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142023-09-0125965565910.22074/cellj.2023.1999800.1278705505Generation of Mouse Model of Hemophilia A by Introducing Novel Mutations, Using CRISPR/Nickase Gene Targeting SystemMehdi Shamsara0Abbas Jamshidizad1Aidin Rahim-Tayefeh2Maliheh Davari3Ali Rajabi Zangi4Fatemeh Masoumi5Alireza Zomorodipour6Department of Animal Biotechnology, Institute Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, IranDepartment of Animal Biotechnology, Institute Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, IranDepartment of Animal Biotechnology, Institute Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, IranDepartment of Molecular Medicine, Institute of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, IranDepartment of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Molecular Medicine, Institute of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, IranDepartment of Molecular Medicine, Institute of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, IranDeveloping mouse models of hemophilia A has been shown to facilitate in vivo studies to explore the probablemechanism(s) underlying the disease and to examine the efficiency of the relevant potential therapeutics. This studyaimed to knockout (KO) the coagulation factor viii (fviii) gene in NMRI mice, using CRISPR/Cas9 (D10A/nickase) system,to generate a mouse model of hemophilia A. Two single guide RNAs (sgRNAs), designed from two distinct regions onNMRI mouse FVIII (mFVIII) exon 3, were designed and inserted in the pX335 vector, expressing both sgRNAs andnickase. The recombinant construct was delivered into mouse zygotes and implanted into the pseudopregnant femalemice’s uterus. Mutant mice were identified by genotyping, genomic sequencing, and mFVIII activity assessment. Twoseparate lines of hemophilia A were obtained through interbreeding the offspring of the female mice receiving potentialCRISPR-Cas9-edited zygotes. Genomic DNA analysis revealed disruptions of the mfviii gene reading frame througha 22-bp deletion and a 23-bp insertion in two separate founder mice. The founder mice showed all the clinical signsof hemophilia A including; excessive bleeding after injuries, and spontaneous bleeding in joints and other organs.Coagulation test data showed that mFVIII coagulation activity was significantly diminished in the mFVIII knockout(FVIIIKO) mice compared to normal mice. The CRISPR/nickase system was successfully applied to generate mouselines with the knockout fviii gene. The two novel FVIIIKO mice demonstrated all clinical symptoms of hemophilia A, whichcould be successfully inherited. Therefore, both of the developed FVIIIKO mouse lines are eligible for being consideredas proper mouse models of hemophilia A for in vivo therapeutic studies.https://www.celljournal.org/article_705505_27aeb300fbfc88bb89c5dce7a7ca5d3a.pdfcrispr-cas9factor viiihemophilia aknockout genemouse models |
| spellingShingle | Mehdi Shamsara Abbas Jamshidizad Aidin Rahim-Tayefeh Maliheh Davari Ali Rajabi Zangi Fatemeh Masoumi Alireza Zomorodipour Generation of Mouse Model of Hemophilia A by Introducing Novel Mutations, Using CRISPR/Nickase Gene Targeting System Cell Journal crispr-cas9 factor viii hemophilia a knockout gene mouse models |
| title | Generation of Mouse Model of Hemophilia A by Introducing Novel Mutations, Using CRISPR/Nickase Gene Targeting System |
| title_full | Generation of Mouse Model of Hemophilia A by Introducing Novel Mutations, Using CRISPR/Nickase Gene Targeting System |
| title_fullStr | Generation of Mouse Model of Hemophilia A by Introducing Novel Mutations, Using CRISPR/Nickase Gene Targeting System |
| title_full_unstemmed | Generation of Mouse Model of Hemophilia A by Introducing Novel Mutations, Using CRISPR/Nickase Gene Targeting System |
| title_short | Generation of Mouse Model of Hemophilia A by Introducing Novel Mutations, Using CRISPR/Nickase Gene Targeting System |
| title_sort | generation of mouse model of hemophilia a by introducing novel mutations using crispr nickase gene targeting system |
| topic | crispr-cas9 factor viii hemophilia a knockout gene mouse models |
| url | https://www.celljournal.org/article_705505_27aeb300fbfc88bb89c5dce7a7ca5d3a.pdf |
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