Isolating mononuclear cells from fetal bone and liver for metabolic, functional, and immunophenotypic analyses in nonhuman primates

Isolating mononuclear cells from fetal bone and liver for metabolic, functional, and immunophenotypic analyses in nonhuman primates

Summary: Studying fetal hematopoiesis is challenging as hematopoiesis transitions from the liver to bone marrow. Obtaining human samples is not possible, and small animal models may not provide sufficient biological material. Here, we present a protocol for isolating hematopoietic cells from the non...

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Bibliographic Details
Main Authors: Michael J. Nash, Evgenia Dobrinskikh, Dong Wang, Eric M. Pietras, Rachel C. Janssen, Jacob E. Friedman, Stephanie R. Wesolowski
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Cell-based Assays
Immunology
Metabolism
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000145

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http://www.sciencedirect.com/science/article/pii/S2666166724000145

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