Measuring external primary cell wall elasticity of seedling roots using atomic force microscopy
Summary: Stiffness plays a central action in plant cell extension. Here, we present a protocol to detect changes in stiffness on the external epidermal cell wall of living plant roots using atomic force microscopy (AFM). We provide generalized instructions for collecting force-distance curves and an...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672300223X |
| _version_ | 1797824486585663488 |
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| author | Harinderbir Kaur Jean-Marie Teulon Anne-Emmanuelle Foucher Daphna Fenel Shu-wen W. Chen Christian Godon Thierry Desnos Jean-Luc Pellequer |
| author_facet | Harinderbir Kaur Jean-Marie Teulon Anne-Emmanuelle Foucher Daphna Fenel Shu-wen W. Chen Christian Godon Thierry Desnos Jean-Luc Pellequer |
| author_sort | Harinderbir Kaur |
| collection | DOAJ |
| description | Summary: Stiffness plays a central action in plant cell extension. Here, we present a protocol to detect changes in stiffness on the external epidermal cell wall of living plant roots using atomic force microscopy (AFM). We provide generalized instructions for collecting force-distance curves and analysis of stiffness using contact-based mechanical model. With this protocol, and some initial training in AFM, a user is able to perform indentation experiments on 4- and 5-day-old Arabidopsis thaliana and determine stiffness properties.For complete details on the use and execution of this protocol, please refer to Godon et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-13T10:39:42Z |
| format | Article |
| id | doaj.art-143d60598c714a4f9456e4cf6cc7885b |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-13T10:39:42Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-143d60598c714a4f9456e4cf6cc7885b2023-05-18T04:40:16ZengElsevierSTAR Protocols2666-16672023-06-0142102265Measuring external primary cell wall elasticity of seedling roots using atomic force microscopyHarinderbir Kaur0Jean-Marie Teulon1Anne-Emmanuelle Foucher2Daphna Fenel3Shu-wen W. Chen4Christian Godon5Thierry Desnos6Jean-Luc Pellequer7Univ. Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, FranceUniv. Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, FranceUniv. Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, FranceUniv. Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, FranceUniv. Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, France; Rue Cyprien Jullin, 38470 Vinay, FranceAix Marseille Université, CNRS, CEA, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA Cadarache, 13115 Saint-Paul lez-Durance, FranceAix Marseille Université, CNRS, CEA, Institut de Biosciences et Biotechnologies Aix-Marseille, Equipe Bioénergies et Microalgues, CEA Cadarache, 13115 Saint-Paul-lez-Durance, France; Corresponding authorUniv. Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, France; Corresponding authorSummary: Stiffness plays a central action in plant cell extension. Here, we present a protocol to detect changes in stiffness on the external epidermal cell wall of living plant roots using atomic force microscopy (AFM). We provide generalized instructions for collecting force-distance curves and analysis of stiffness using contact-based mechanical model. With this protocol, and some initial training in AFM, a user is able to perform indentation experiments on 4- and 5-day-old Arabidopsis thaliana and determine stiffness properties.For complete details on the use and execution of this protocol, please refer to Godon et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672300223XAtomic Force Microscopy (AFM)Plant SciencesPhysics |
| spellingShingle | Harinderbir Kaur Jean-Marie Teulon Anne-Emmanuelle Foucher Daphna Fenel Shu-wen W. Chen Christian Godon Thierry Desnos Jean-Luc Pellequer Measuring external primary cell wall elasticity of seedling roots using atomic force microscopy STAR Protocols Atomic Force Microscopy (AFM) Plant Sciences Physics |
| title | Measuring external primary cell wall elasticity of seedling roots using atomic force microscopy |
| title_full | Measuring external primary cell wall elasticity of seedling roots using atomic force microscopy |
| title_fullStr | Measuring external primary cell wall elasticity of seedling roots using atomic force microscopy |
| title_full_unstemmed | Measuring external primary cell wall elasticity of seedling roots using atomic force microscopy |
| title_short | Measuring external primary cell wall elasticity of seedling roots using atomic force microscopy |
| title_sort | measuring external primary cell wall elasticity of seedling roots using atomic force microscopy |
| topic | Atomic Force Microscopy (AFM) Plant Sciences Physics |
| url | http://www.sciencedirect.com/science/article/pii/S266616672300223X |
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