Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara)
Abstract Ammonia is an essential substance for agriculture and the chemical industry. The intracellular production of ammonia in yeast (Saccharomyces cerevisiae) by metabolic engineering is difficult because yeast strongly assimilates ammonia, and the knockout of genes enabling this assimilation is...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2020-04-01
|
| Series: | AMB Express |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13568-020-01011-9 |
| _version_ | 1818306153707208704 |
|---|---|
| author | Yukio Watanabe Kouichi Kuroda Yuki Tatemichi Takeharu Nakahara Wataru Aoki Mitsuyoshi Ueda |
| author_facet | Yukio Watanabe Kouichi Kuroda Yuki Tatemichi Takeharu Nakahara Wataru Aoki Mitsuyoshi Ueda |
| author_sort | Yukio Watanabe |
| collection | DOAJ |
| description | Abstract Ammonia is an essential substance for agriculture and the chemical industry. The intracellular production of ammonia in yeast (Saccharomyces cerevisiae) by metabolic engineering is difficult because yeast strongly assimilates ammonia, and the knockout of genes enabling this assimilation is lethal. Therefore, we attempted to produce ammonia outside the yeast cells by displaying a glutaminase (YbaS) from Escherichia coli on the yeast cell surface. YbaS-displaying yeast successfully produced 3.34 g/L ammonia from 32.6 g/L glutamine (83.2% conversion rate), providing it at a higher yield than in previous studies. Next, using YbaS-displaying yeast, we also succeeded in producing ammonia from glutamine in soybean residues (okara) produced as food waste from tofu production. Therefore, ammonia production outside cells by displaying ammonia-lyase on the cell surface is a promising strategy for producing ammonia from food waste as a novel energy resource, thereby preventing food loss. |
| first_indexed | 2024-12-13T06:37:58Z |
| format | Article |
| id | doaj.art-14403a4c908645a19116348a85c5eeff |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-13T06:37:58Z |
| publishDate | 2020-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-14403a4c908645a19116348a85c5eeff2022-12-21T23:56:29ZengSpringerOpenAMB Express2191-08552020-04-011011810.1186/s13568-020-01011-9Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara)Yukio Watanabe0Kouichi Kuroda1Yuki Tatemichi2Takeharu Nakahara3Wataru Aoki4Mitsuyoshi Ueda5Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto UniversityDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto UniversityDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto UniversityResearch & Development Division, Kikkoman CorporationDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto UniversityDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto UniversityAbstract Ammonia is an essential substance for agriculture and the chemical industry. The intracellular production of ammonia in yeast (Saccharomyces cerevisiae) by metabolic engineering is difficult because yeast strongly assimilates ammonia, and the knockout of genes enabling this assimilation is lethal. Therefore, we attempted to produce ammonia outside the yeast cells by displaying a glutaminase (YbaS) from Escherichia coli on the yeast cell surface. YbaS-displaying yeast successfully produced 3.34 g/L ammonia from 32.6 g/L glutamine (83.2% conversion rate), providing it at a higher yield than in previous studies. Next, using YbaS-displaying yeast, we also succeeded in producing ammonia from glutamine in soybean residues (okara) produced as food waste from tofu production. Therefore, ammonia production outside cells by displaying ammonia-lyase on the cell surface is a promising strategy for producing ammonia from food waste as a novel energy resource, thereby preventing food loss.http://link.springer.com/article/10.1186/s13568-020-01011-9AmmoniaCell surface engineeringSaccharomyces cerevisiaeGlutaminaseFood waste |
| spellingShingle | Yukio Watanabe Kouichi Kuroda Yuki Tatemichi Takeharu Nakahara Wataru Aoki Mitsuyoshi Ueda Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara) AMB Express Ammonia Cell surface engineering Saccharomyces cerevisiae Glutaminase Food waste |
| title | Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara) |
| title_full | Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara) |
| title_fullStr | Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara) |
| title_full_unstemmed | Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara) |
| title_short | Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara) |
| title_sort | construction of engineered yeast producing ammonia from glutamine and soybean residues okara |
| topic | Ammonia Cell surface engineering Saccharomyces cerevisiae Glutaminase Food waste |
| url | http://link.springer.com/article/10.1186/s13568-020-01011-9 |
| work_keys_str_mv | AT yukiowatanabe constructionofengineeredyeastproducingammoniafromglutamineandsoybeanresiduesokara AT kouichikuroda constructionofengineeredyeastproducingammoniafromglutamineandsoybeanresiduesokara AT yukitatemichi constructionofengineeredyeastproducingammoniafromglutamineandsoybeanresiduesokara AT takeharunakahara constructionofengineeredyeastproducingammoniafromglutamineandsoybeanresiduesokara AT wataruaoki constructionofengineeredyeastproducingammoniafromglutamineandsoybeanresiduesokara AT mitsuyoshiueda constructionofengineeredyeastproducingammoniafromglutamineandsoybeanresiduesokara |